Kholkute S D, Katkam R R, Nandedkar T D, Puri C P
Primate Biology Division, Institute for Research in Reproduction (ICMR), Jehangir Merwanji Street, Parel, Mumbai 400 012, India.
Mol Hum Reprod. 2000 Apr;6(4):337-43. doi: 10.1093/molehr/6.4.337.
In the present study, changes in the immunohistochemical localization of leukaemia inhibitory factor (LIF) in the endometrium during various phases of ovarian cyclicity of the common marmoset have been reported. LIF was absent during the early and late follicular phases. LIF was observed mainly in the cytoplasm of the endometrial glands during the early luteal phase, reached maximum intensity during the mid-luteal phase and declined again during late luteal phase. In-situ hybridization also showed a similar cyclic pattern in the expression of LIF. Stromal cells only showed signals for LIF during the mid-luteal phase. In ovariectomized marmosets, graded dosages of oestradiol alone failed to induce the appearance of LIF protein. Progesterone treatment following oestradiol priming, however, induced distinct glandular localization of LIF, indicating that LIF is a progesterone-dependent protein. Thus endometrial LIF is under maternal control and is secreted in response to the increased progesterone concentrations in circulation. It is possible that high concentrations of LIF during mid-luteal phase may prepare the endometrium for blastocyst implantation in marmosets.
在本研究中，已经报道了普通狨猴卵巢周期性各阶段子宫内膜中白血病抑制因子（LIF）免疫组化定位的变化。在卵泡早期和晚期不存在LIF。在黄体早期，LIF主要在子宫内膜腺体的细胞质中观察到，在黄体中期达到最大强度，在黄体晚期再次下降。原位杂交也显示LIF表达有类似的周期性模式。基质细胞仅在黄体中期显示LIF信号。在去卵巢的狨猴中，单独给予不同剂量的雌二醇未能诱导LIF蛋白出现。然而，在雌二醇预处理后给予孕酮治疗，可诱导LIF在腺体中明显定位，表明LIF是一种孕酮依赖性蛋白。因此，子宫内膜LIF受母体控制，并随着循环中孕酮浓度的增加而分泌。黄体中期高浓度的LIF可能为狨猴的囊胚着床准备子宫内膜。
