Verloo D, Brandt J, Van Meirvenne N, Büscher P
Department of Parasitology, Institute of Tropical Medicine, Nationalestraat 155, B-2000, Antwerp, Belgium.
Vet Parasitol. 2000 Mar 28;89(1-2):129-32. doi: 10.1016/s0304-4017(00)00191-6.
Ten blood samples randomly collected from cows on a farm nearby Antwerp, Belgium, were inoculated into KIVI culture medium (Kit for In Vitro Isolation of trypanosomes) and RPMI 10%+feeder medium. Within 3 weeks of incubation all KIVI cultures and four RPMI 10%+feeder revealed presence of Trypanosoma theileri. Some practical implications regarding the use of KIVI for isolation of pathogenic African trypanosomes from cattle and other Bovidae are discussed.
从比利时安特卫普附近一个农场的奶牛身上随机采集了10份血样，接种到KIVI培养基（锥虫体外分离试剂盒）和RPMI 10% +饲养层培养基中。在培养3周内，所有KIVI培养物和4份RPMI 10% +饲养层培养基中均发现了泰勒锥虫。讨论了关于使用KIVI从牛和其他牛科动物中分离致病性非洲锥虫的一些实际意义。
