Williams-Bouyer N, Reisner B S, Woods G L
University of Texas Medical Branch at Galveston, Department of Pathology, 301 University Boulevard, Galveston, TX 77555-0740, USA.
Diagn Microbiol Infect Dis. 2000 Mar;36(3):159-62. doi: 10.1016/s0732-8893(99)00146-7.
The performance of the AccuProbe Group B Streptococcus Culture Identification Test (Gen-Probe Incorporated, San Diego, CA, USA) for the detection of group B streptococci (GBS) directly from LIM broth cultures of vaginal-anorectal swab specimens from pregnant women (two swabs per patient in most cases) was evaluated by comparing results to those of conventional GBS culture. Of 411 specimens analyzed, 82 were positive and 312 were negative for GBS by both methods. After initial testing, the percent agreement was 95.9%. The initial sensitivity, specificity, and positive and negative predictive values for the AccuProbe test were 90.1%, 97.5%, 91.1%, and 97.2%, respectively. Results were discrepant for 17 specimens: eight were GBS positive by probe and negative by culture; nine were negative by probe and positive by culture. To resolve discrepancies, culture plates were re-examined for GBS colonies, AccuProbe testing was repeated on the initial LIM broth cultures, and the second swab (if received) was inoculated to LIM broth for AccuProbe testing after overnight incubation. After discrepant resolution testing, the percent agreement between the two test methods was 97.8%. The final sensitivity, specificity, and positive and negative predictive values for the AccuProbe test were 95.6%, 98.4%, 94.6%, and 98.7%, respectively. These data suggest that the AccuProbe test is a reliable method for detecting GBS in vaginal-anorectal specimens, providing results more rapidly than conventional culture. However, strict adherence to the manufacturer's test protocol is necessary to limit technical errors.
通过将结果与传统B族链球菌（GBS）培养结果进行比较，评估了AccuProbe B族链球菌培养鉴定试验（美国加利福尼亚州圣地亚哥的Gen-Probe公司）直接从孕妇阴道-直肠拭子标本的LIM肉汤培养物中检测GBS的性能（大多数情况下每位患者采集两支拭子）。在分析的411份标本中，两种方法检测GBS的结果均为82份阳性，312份阴性。初始检测后，一致性百分比为95.9%。AccuProbe试验的初始敏感性、特异性、阳性预测值和阴性预测值分别为90.1%、97.5%、91.1%和97.2%。17份标本的结果存在差异：8份通过探针检测为GBS阳性而培养检测为阴性；9份通过探针检测为阴性而培养检测为阳性。为解决差异，重新检查培养平板以寻找GBS菌落，对初始LIM肉汤培养物重复进行AccuProbe检测，并将第二支拭子（如果收到）接种到LIM肉汤中，过夜培养后进行AccuProbe检测。差异解决检测后，两种检测方法之间的一致性百分比为97.8%。AccuProbe试验的最终敏感性、特异性、阳性预测值和阴性预测值分别为95.6%、98.4%、94.6%和98.7%。这些数据表明，AccuProbe试验是检测阴道-直肠标本中GBS的可靠方法，比传统培养能更快地提供结果。然而，必须严格遵守制造商的试验方案以限制技术误差。
