Mohammed O B, Davies A J, Hussein H S, Daszak P
King Khalid Wildlife Research Centre, Thumamah, National Commission for Wildlife Conservation and Development, Riyadh, Saudi Arabia.
Vet Rec. 2000 Feb 19;146(8):218-21. doi: 10.1136/vr.146.8.218.
The prevalence of Sarcocystis species in muscle samples from gazelles kept as breeding groups at the King Khalid Wildlife Research Centre, Saudi Arabia, was determined by fibreoptic examination, pepsin digestion and histological techniques. No macroscopic sarcocysts were detected by fibreoptic examination, and the overall prevalence of Sarcocystis was 66 x 7 per cent by pepsin digestion, and 39 x 9 per cent by histological examination. By digestion, the tongue contained the highest density of bradyzoites in Gazella dorcas, and Gazella gazella erlangeri, the oesophagus in Gazella subgutturosa marica and skeletal muscle in Gazella gazella and Gazella thomsoni. Skeletal muscle was least affected in G dorcas, the oesophagus in G gazella, and the diaphragm in G g erlangeri, G s marica and G thomsoni. By histology, the heart contained most microcysts, except in G g erlangeri, in which the tongue was most affected. No single tissue type was therefore suitable for the diagnosis of sarcocystosis in this multispecies collection, although digestion was more sensitive in detecting infection than histology. The level of Sarcocystis infection was significantly higher in free-ranging gazelles kept in a main enclosure than in gazellas kept in breeding pens, and higher in adult gazelles than in juveniles.
通过纤维光学检查、胃蛋白酶消化和组织学技术，确定了沙特阿拉伯哈利德国王野生动物研究中心作为繁殖群体饲养的瞪羚肌肉样本中肉孢子虫属的流行情况。纤维光学检查未检测到肉眼可见的肉孢子囊，通过胃蛋白酶消化法检测到的肉孢子虫总体流行率为66×7%，通过组织学检查为39×9%。通过消化法发现，在多卡瞪羚、厄氏瞪羚中，舌头所含缓殖子密度最高；在鹅喉羚中，食管所含缓殖子密度最高；在细角瞪羚和汤氏瞪羚中，骨骼肌所含缓殖子密度最高。在多卡瞪羚中，骨骼肌受影响最小；在细角瞪羚中，食管受影响最小；在厄氏瞪羚、鹅喉羚和汤氏瞪羚中，膈肌受影响最小。通过组织学检查发现，除厄氏瞪羚外，心脏所含微囊肿最多，在厄氏瞪羚中，舌头受影响最大。因此，在这个多物种样本中，没有单一的组织类型适合用于肉孢子虫病的诊断，不过消化法在检测感染方面比组织学检查更敏感。在主围栏中自由放养的瞪羚的肉孢子虫感染水平显著高于在繁殖栏中饲养的瞪羚，成年瞪羚的感染水平高于幼年瞪羚。
