LeBelle M, Graham K, Wilson W L
J Pharm Sci. 1979 May;68(5):555-6. doi: 10.1002/jps.2600680509.
A rapid high-performance liquid chromatographic assay for penicillin V content of penicillin V benzathine bulk drug and oral suspensions is described. Dilution of the oral suspension with methanol containing 1,3,5-trimethoxybenzene as the internal standard allowed for direct analysis on a reversed-phase column with a mobile phase of 53% methanol in 0.05 M aqueous pH 3.5 phosphate buffer. A relative standard deviation of less than 1% was obtained on commercial formulations, and the system was linear over a range 10-40 microgram injected.
描述了一种用于测定苄星青霉素原料药和口服混悬液中青霉素V含量的快速高效液相色谱法。用含有1,3,5 -三甲氧基苯作为内标的甲醇稀释口服混悬液，可在反相柱上直接分析，流动相为含53%甲醇的0.05 M pH 3.5磷酸盐缓冲水溶液。在市售制剂上获得的相对标准偏差小于1%，该系统在进样量10 - 40微克范围内呈线性。
