Smith M P, Mathur R S, Keay S D, Hall L, Hull M G, Jenkins J M
University of Bristol and St. Michaels Hospital, Bristol, United Kingdom.
Fertil Steril. 2000 Apr;73(4):825-30. doi: 10.1016/s0015-0282(99)00607-x.
To further elucidate cortisol metabolism in the follicular microenvironment at the time of oocyte retrieval, the presence of 11beta-hydroxysteroid dehydrogenase (HSD) messenger (m)RNA transcripts in oocytes; cumulus cells; granulosa cells; and CD45(+), CD15(+) leukocytes was assessed semiquantitatively.
Controlled study using semiquantitative assessment of 11beta-HSD mRNA.
University IVF center.
PATIENT(S): Twenty-six patients undergoing controlled ovarian hyperstimulation for assisted conception.
INTERVENTION(S): Metaphase II oocytes; cumulus cells; granulosa cells, and CD45(+), CD15(+) leukocytes from individual follicular fluid aspirates.
Semiquantitative analysis of PCR products after total RNA extraction and complementary DNA synthesis.
RESULT(S): Periovulatory human oocytes; cumulus cells; CD45(+), CD15(+) leukocytes; and granulosa cells consistently express type 1 but not type 2 11beta-HSD mRNA. Expression of mRNA is greatest in cumulus cells. Type 1 11beta-HSD mRNA expression varies considerably in all cell types and among individual follicles and patients.
CONCLUSION(S): These studies of mRNA expression suggest that the enzymes present both in and around the periovulatory oocyte will favor a high-cortisol environment.
为了进一步阐明取卵时卵泡微环境中的皮质醇代谢情况，对卵母细胞、卵丘细胞、颗粒细胞以及CD45(+)、CD15(+)白细胞中11β-羟类固醇脱氢酶（HSD）信使核糖核酸（mRNA）转录本的存在情况进行了半定量评估。
采用对11β-HSD mRNA进行半定量评估的对照研究。
大学体外受精中心。
26名接受控制性卵巢过度刺激以进行辅助受孕的患者。
来自单个卵泡液抽吸物中的中期II卵母细胞、卵丘细胞、颗粒细胞以及CD45(+)、CD15(+)白细胞。
总RNA提取和互补DNA合成后对PCR产物进行半定量分析。
排卵周围的人类卵母细胞、卵丘细胞、CD45(+)、CD15(+)白细胞以及颗粒细胞持续表达1型而非2型11β-HSD mRNA。mRNA的表达在卵丘细胞中最高。1型11β-HSD mRNA的表达在所有细胞类型以及各个卵泡和患者之间差异很大。
这些mRNA表达研究表明，排卵周围卵母细胞内外存在的酶将有利于形成高皮质醇环境。
