Evagelatou M, Peterson S L, Cooke B A
Department of Biochemistry and Molecular Biology, Royal Free Hospital School of Medicine, University of London, UK.
Mol Cell Endocrinol. 1997 Oct 20;133(2):81-8. doi: 10.1016/s0303-7207(97)00146-9.
It is well established that there are interactions between the immune and reproductive systems. The ovary contains indigenous macrophages, as well as other classes of leukocytes in smaller numbers. Cytokines secreted by these cells have been shown to have the ability to regulate ovarian steroidogenesis. In the present study, the effect of leukocytes on 11beta-hydroxysteroid dehydrogenase (11beta-HSD) in human granulosa-lutein cells was examined. In addition, individual cytokines were also tested for their ability to regulate this enzyme. The follicular aspirates of patients undergoing IVF treatment were used as a source of granulosa cells. Cells isolated from these aspirates were found to contain between 15 and 60% leukocytes as assessed by flow cytometry (FACS). Leukocytes were removed from the sample preparations by the use of immunomagnetic beads coated with CD45 antibody, which recognises a surface antigen on all classes of leukocyte. Removal of leukocytes significantly decreased the 11beta-HSD activity in the granulosa cells, assayed after 3 days of culture, from 7.3 (2-20) to 3.5 (1-10) pmol cortisone formed/50000 cells/4 h (medians and ranges, n = 15). Addition of IL-5 and IL-6 significantly increased the 11beta-HSD activity in granulosa cell cultures both in the presence and absence of leukocytes. Addition of IL-4 and IFN-gamma increased 11beta-HSD activity only in the leukocyte-depleted granulosa cell cultures, whereas IL-2 had no effect on either of the cultures. The data suggests that leukocytes interact with the ovarian cells through cytokine secretion and/or cell-cell contact to increase the 11beta-HSD activity in human granulosa cells.
免疫和生殖系统之间存在相互作用，这一点已得到充分证实。卵巢中含有天然巨噬细胞以及数量较少的其他类型白细胞。这些细胞分泌的细胞因子已被证明具有调节卵巢类固醇生成的能力。在本研究中，检测了白细胞对人颗粒黄体细胞中11β-羟类固醇脱氢酶（11β-HSD）的影响。此外，还测试了单个细胞因子调节该酶的能力。接受体外受精治疗患者的卵泡抽吸物用作颗粒细胞的来源。通过流式细胞术（FACS）评估，从这些抽吸物中分离出的细胞含有15%至60%的白细胞。通过使用包被有CD45抗体的免疫磁珠从样品制剂中去除白细胞，CD45抗体可识别所有类型白细胞上的表面抗原。去除白细胞后，培养3天后检测的颗粒细胞中11β-HSD活性从7.3（2 - 20）显著降低至3.5（1 - 10）pmol皮质酮形成/50000细胞/4小时（中位数和范围，n = 15）。添加IL-5和IL-6在有或无白细胞存在的情况下均显著增加了颗粒细胞培养物中的11β-HSD活性。添加IL-4和IFN-γ仅在去除白细胞的颗粒细胞培养物中增加了11β-HSD活性，而IL-2对两种培养物均无影响。数据表明，白细胞通过细胞因子分泌和/或细胞间接触与卵巢细胞相互作用，以增加人颗粒细胞中的11β-HSD活性。
