Barbosa H M, Silva M, Ferro E A, Mineo J R
Universidade Federal de Uberlândia, Uberlândia, MG, 38400-902, Brasil.
Mem Inst Oswaldo Cruz. 2000 Mar-Apr;95(2):265-70. doi: 10.1590/S0074-02762000000200023.
Infection of non-adherent TG180 murine sarcoma cells with Toxoplasma gondii was compared, at the ultrastructural level, in both in vivo and in vitro conditions. Suspensions of 3.0 x 10(6) TG180 cells infected in vitro with 1.0 x 10(6) parasites of the RH strain were harvested between the first and 6th day post-infection and processed for transmission electron microscopy. In vivo infection was made by intraperitoneal inoculation in mice of 1.0 x 10(6) TG180 cells, that were co-inoculated with a parasite suspension at the same cell concentration. Cells were harvested 10, 20, 30 min and 24, 48 h post-inoculation and processed for transmission electron microscopy at the same conditions of the in vitro culture. It was observed TG180 murine sarcoma cells with intense and equivalent intracellular parasitism in both conditions. Host cells with parasitophorous vacuoles containing up to 16 parasites, as well as parasites undergoing mitoses or presenting a bradyzoite-like morphology, were frequently seen in both culture methods.
在体内和体外条件下，在超微结构水平上比较了刚地弓形虫对非贴壁TG180小鼠肉瘤细胞的感染情况。在感染后第1天至第6天收获体外感染了1.0×10⁶ RH株寄生虫的3.0×10⁶ TG180细胞悬液，并进行透射电子显微镜处理。体内感染是通过向小鼠腹腔接种1.0×10⁶ TG180细胞，并以相同细胞浓度同时接种寄生虫悬液来进行的。在接种后10、20、30分钟以及24、48小时收获细胞，并在与体外培养相同的条件下进行透射电子显微镜处理。在两种条件下均观察到TG180小鼠肉瘤细胞存在强烈且等效的细胞内寄生现象。在两种培养方法中均经常见到含有多达16个寄生虫的寄生泡的宿主细胞，以及正在进行有丝分裂或呈现类缓殖子形态的寄生虫。
