Pope C E
Audubon Institute Center for Research of Endangered Species, New Orleans, Louisiana 70131, USA.
Theriogenology. 2000 Jan 1;53(1):163-74. doi: 10.1016/s0093-691x(99)00249-6.
Most of the 36 species of wild cats are classified as threatened, vulnerable or endangered due to poaching and habitat loss. The important role of assisted reproduction techniques (ART) as part of a multifaceted captive breeding program for selected wild cat species is gradually gaining acceptance. This recognition is a result of the progress made during the last decade in which the feasibility of oocyte recovery from gonadotropin-treated females, in vitro fertilization, embryo cryopreservation and embryo transfer (ET) was demonstrated in the domestic cat (Felis catus). Additionally, embryos have been produced in vitro from oocytes matured in vitro after recovery from ex situ ovaries of both domestic and non-domestic cat species and domestic kittens have been born following transfer of these embryos. In vitro fertilization has been successful in at least one-third of wild cat species and kittens were born after transfer of Indian desert cat (Felis sylvestris ornata) embryos into a domestic cat and con-specific transfer of tiger (Panthera tigris) embryos. The domestic cat is not only a valuable model for development of in vitro techniques but may serve as a recipient of embryos from several species of small wild cats.
36种野生猫科动物中的大多数由于偷猎和栖息地丧失而被列为受威胁、易危或濒危物种。辅助生殖技术（ART）作为针对特定野生猫科动物的多方面圈养繁殖计划的一部分，其重要作用正逐渐得到认可。这种认可源于过去十年所取得的进展，在此期间，在家猫（Felis catus）身上证明了从经促性腺激素处理的雌性动物回收卵母细胞、体外受精（IVF）、胚胎冷冻保存及胚胎移植（ET）的可行性。此外，从家猫和非家猫物种的异地卵巢回收的卵母细胞经体外成熟后，已在体外产生胚胎，并且在移植这些胚胎后诞生了家猫幼崽。体外受精在至少三分之一的野生猫科动物中取得成功，印度沙漠猫（Felis sylvestris ornata）胚胎移植到家猫体内以及老虎（Panthera tigris）胚胎同种移植后诞生了幼崽。家猫不仅是体外技术开发的宝贵模型，还可作为几种小型野生猫科动物胚胎的受体。
