Wijayagunawardane M P, Miyamoto A, Sato K
Department of Animal Science, University of Peradeniya, Sri Lanka.
Theriogenology. 1999 Oct 1;52(5):791-801. doi: 10.1016/S0093-691X(99)00172-7.
The optimal oviductal environment, including contractile activity for gamete transport, fertilization and early embryonic development, is mediated by physiological and anatomical changes in the oviduct during the estrous cycle. Oviductal epithelial cell culture was utilized to investigate the effect of ovarian steroids (progesterone [P4] and estradiol 17 beta [E2]), oxytocin (OT) and luteinizing hormone (LH) on the local production of prostaglandin E2 (PGE2), prostaglandin F2 alpha (PGF2 alpha) and endothelin-1 (ET-1) in the cow oviduct. Epithelial cells isolated from oviducts collected during the follicular phase were cultured in M199 under standard culture conditions until monolayer formation. Then the cells were trypsinized and plated at a density of 3 x 10(4)/mL/well and cultured again until subconfluency, at which time the cells were incubated for 4 or 24 h with M199 only (control), high P4 (H-P4; 1 microgram/mL), low P4 (L-P4; 10 ng/mL), E2 (1 ng/mL), LH (10 ng/mL), OT (10(-9) M) ET-1 (10(-9) M), PGE2 (10(-8) M) PGF2 alpha (10(-9) M) or their combination (H-P4 + E2, L-P4 + E2, LH + E2, ET-1 + E2, L-P4 + E2 + LH and H-P4 + E2 + LH). The production of both PG and ET-1 was increased by E2 + low P4 and LH + E2 + low P4 (P < 0.05), while LH + E2 enhanced the production of PGF2 alpha and ET-1 (P < 0.05). Moreover, E2 + ET-1 stimulated PG production (P < 0.05). However, OT had no effect on the production of any of these substances. These results suggest that the preovulatory LH surge, together with locally re-circulated high levels of E2 from the Graafian follicle and basal P4 from regressing corpus luteum (CL), induces the maximum stimulatory effect on oviductal PGE2, PGF2 alpha and ET-1 production during the periovulatory period. Consequently, the elevated local ET-1 concentration during periovulatory period may induce the high contractile activity of the oviduct and, at the same time, the stimulation of PG production. Thus, ET-1 may act as a local amplifier for oviductal PG production stimulated by LH and ovarian steroids.
最佳输卵管环境，包括配子运输、受精和早期胚胎发育的收缩活动，是由发情周期中输卵管的生理和解剖变化介导的。利用输卵管上皮细胞培养来研究卵巢类固醇（孕酮[P4]和17β-雌二醇[E2]）、催产素（OT）和促黄体生成素（LH）对奶牛输卵管中前列腺素E2（PGE2）、前列腺素F2α（PGF2α）和内皮素-1（ET-1）局部产生的影响。从卵泡期采集的输卵管中分离出的上皮细胞在标准培养条件下于M199中培养，直至形成单层。然后将细胞用胰蛋白酶消化，以3×10⁴/mL/孔的密度接种并再次培养，直至接近汇合，此时细胞分别用仅含M199（对照）、高P4（H-P4；1μg/mL）、低P4（L-P4；10ng/mL）、E2（1ng/mL）、LH（10ng/mL）、OT（10⁻⁹M）、ET-1（10⁻⁹M）、PGE2（10⁻⁸M）、PGF2α（10⁻⁹M）或它们的组合（H-P4 + E2、L-P4 + E2、LH + E2、ET-1 + E2、L-P4 + E2 + LH和H-P4 + E2 + LH）孵育4或24小时。E2 + 低P4以及LH + E2 + 低P4可增加PG和ET-1的产生（P < 0.05），而LH + E2可增强PGF2α和ET-1的产生（P < 0.05）。此外，E2 + ET-1刺激PG产生（P < 0.05）。然而，OT对这些物质中的任何一种产生均无影响。这些结果表明，排卵前LH峰，连同来自格拉夫卵泡局部再循环的高水平E2和退化黄体（CL）的基础P4，在排卵前期对输卵管PGE2、PGF2α和ET-1的产生诱导最大刺激作用。因此，排卵前期局部ET-1浓度升高可能诱导输卵管的高收缩活性，同时刺激PG产生。因此，ET-1可能作为LH和卵巢类固醇刺激输卵管PG产生的局部放大器。
