Chuang Y H, Chuang W L, Chen S S, Huang C H
Department of Neurology, School of Medicine, Kaohsiung Medical College, Taiwan, ROC.
J Urol. 2000 Apr;163(4):1298-303.
Transforming growth factor (TGF-beta) is a multifunctional cytokine that may stimulate fibroblast proliferation and regulate the synthesis of matrix components. To elucidate the role of TGF-beta1 in the process of ureteric fibrosis in obstructive uropathy, we conducted this study.
The expression of TGF-beta1 and TGF-beta receptors I, II, III were studied in 54 rats by the method of immunohistochemistry.
The degree of smooth muscle hypertrophy and serosa thickening aggravated gradually along the course of ureteric ligation. The expression of TGF-beta1 in the serosa layer was noticed from day 7 after ligation. The expression of TGF-beta1 and receptors in the serosa layer progressively increased during the period of ureteric obstruction, reached a peak on day 21 after ligation, and then declined. The expression of TGF-beta1 and TGF-beta receptors (II and III) in the serosa layer was correlated significantly (r = 0.8048 and 0.7974, respectively; p values both <0.0001). The expression of TGF-beta1 also correlated significantly with the thickening of serosa layer and the severity of hydroureter (r = 0.6921 and 0.5394, respectively; p values both <0.005). The expression of TGF-beta1 and TGF-beta receptors in the smooth muscle layer was transient and weak, and the expression of TGF-beta1 and TGF-beta receptors was accompanied with the fibrosis in the muscle layer.
We conclude that TGF-beta1 might participate in the progression ofureteric fibrosis in obstructed ureters.
转化生长因子（TGF-β）是一种多功能细胞因子，可刺激成纤维细胞增殖并调节基质成分的合成。为阐明TGF-β1在梗阻性肾病输尿管纤维化过程中的作用，我们进行了本研究。
采用免疫组织化学方法研究了54只大鼠中TGF-β1及TGF-β受体I、II、III的表达。
输尿管结扎后，平滑肌肥大和浆膜增厚程度沿输尿管走行逐渐加重。结扎后第7天在浆膜层发现TGF-β1的表达。在输尿管梗阻期间，浆膜层中TGF-β1及其受体的表达逐渐增加，在结扎后第21天达到峰值，然后下降。浆膜层中TGF-β1与TGF-β受体（II和III）的表达显著相关（r分别为0.8048和0.7974；p值均<0.0001）。TGF-β1的表达也与浆膜层增厚及输尿管积水严重程度显著相关（r分别为0.6921和0.5394；p值均<0.005）。平滑肌层中TGF-β1及其受体的表达短暂且较弱，TGF-β1及其受体的表达与肌层纤维化相伴。
我们得出结论，TGF-β1可能参与梗阻性输尿管中输尿管纤维化的进展。
