Chuang Yen-Hwang, Chuang Wan-Long, Huang Shu-Pin, Huang Chun-Hsiung
Department of Neurology, Kaohsiung Medical University, Kaohsiung, Taiwan.
Scand J Urol Nephrol. 2005;39(1):7-14. doi: 10.1080/00365590510007649.
To investigate the roles of epidermal growth factor (EGF), basic fibroblast growth factor (bFGF) and insulin growth factor-1 (IGF-1) in the myocyte regeneration of obstructed ureters.
The expression of EGF, bFGF, IGF-1 and proliferation cell nuclear antigen (PCNA) was studied immunohistochemically in 54 female Sprague-Dawley rats.
Tissue damage to the smooth muscle layer in the obstructed ureters was aggravated during the period of obstruction. The expression of EGF, bFGF and IGF-1 in myocytes was detected using the method of concurrent immunohistochemical staining. The expression of EGF, bFGF and IGF-1 in the smooth muscle layer was found from Day 14 after ligation. The expression of EGF, bFGF and IGF-1 increased to a peak on Day 21 and then declined. The expression of PCNA in the smooth muscle layer was also found from Day 14 after ligation and increased to a peak on Day 21. The expressions of EGF, bFGF and IGF-1 were significantly correlated with the expression of PCNA in the smooth muscle layer (r=0.7982, 0.6264 and 0.5840, respectively; p-values all <0.002). Co-expression of EGF, bFGF, IGF-1 and PCNA was determined using the method of double immunofluorescence staining. Co-expression of PCNA was observed in 34% of EGF-positive myocytes, 53.6% of bFGF-positive myocytes and 41.1% of IGF-1-positive myocytes at Day 21 post-ligation.
Expression of EGF, bFGF and IGF-1 may contribute to myocyte regeneration of damaged ureters in rats with obstructive uropathy.
探讨表皮生长因子（EGF）、碱性成纤维细胞生长因子（bFGF）和胰岛素样生长因子-1（IGF-1）在梗阻性输尿管肌细胞再生中的作用。
采用免疫组织化学方法研究54只雌性Sprague-Dawley大鼠中EGF、bFGF、IGF-1和增殖细胞核抗原（PCNA）的表达。
梗阻期间，梗阻性输尿管平滑肌层的组织损伤加重。采用免疫组织化学同步染色法检测肌细胞中EGF、bFGF和IGF-1的表达。结扎后第14天发现平滑肌层中有EGF、bFGF和IGF-1的表达。EGF、bFGF和IGF-1的表达在第21天升至峰值，随后下降。结扎后第14天也发现平滑肌层中有PCNA的表达，并在第21天升至峰值。平滑肌层中EGF、bFGF和IGF-1的表达与PCNA的表达显著相关（r分别为0.7982、0.6264和0.5840；p值均<0.002）。采用双重免疫荧光染色法测定EGF、bFGF、IGF-1和PCNA的共表达。结扎后第21天，在34%的EGF阳性肌细胞、53.6%的bFGF阳性肌细胞和41.1%的IGF-1阳性肌细胞中观察到PCNA的共表达。
EGF、bFGF和IGF-1的表达可能有助于梗阻性肾病大鼠受损输尿管的肌细胞再生。
