• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

用于在乳糖启动子控制下进行基因克隆和表达的噬菌体λ-大肠杆菌K12载体-宿主系统：I. 在lacZ EcoRI限制性位点插入DNA片段。

Bacteriophage lambda-E. coli K12 vector-host system for gene cloning and expression under lactose promoter control: I. DNA fragment insertion at the lacZ EcoRI restriction site.

作者信息

Pourcel C, Marchal C, Louise A, Fritsch A, Tiollais P

出版信息

Mol Gen Genet. 1979 Feb 26;170(2):161-9. doi: 10.1007/BF00337792.

DOI:10.1007/BF00337792
PMID:107392
Abstract

Bacteriophage lambda vectors, derived from lambda plac5 were constructed. Their genomes have only one EcoRI restriction site, located near the end of the beta-galactosidase gene. Recombinants, constructed in vitro, having a DNA fragment inserted in the EcoRI site, are lac- and can be easily recognized. Expression of such foreign genes is then under the control of the lac promoter. Mutations Qam73 and Sam7 greatly increase the amount of beta-galactosidase synthesized by the vector bacteriophage. The lambda ZEQS vector has been certified B2 (EK2) by the French control commission "Recombinaisons génétiques in vitro".

摘要

构建了源自λplac5的噬菌体λ载体。它们的基因组只有一个EcoRI限制性酶切位点，位于β-半乳糖苷酶基因末端附近。在体外构建的、将DNA片段插入EcoRI位点的重组体是乳糖阴性的，并且易于识别。此类外源基因的表达随后受乳糖启动子的控制。突变Qam73和Sam7极大地增加了载体噬菌体合成的β-半乳糖苷酶的量。λZEQS载体已被法国“体外基因重组”控制委员会认证为B2（EK2）。

相似文献

1
Bacteriophage lambda-E. coli K12 vector-host system for gene cloning and expression under lactose promoter control: I. DNA fragment insertion at the lacZ EcoRI restriction site.用于在乳糖启动子控制下进行基因克隆和表达的噬菌体λ-大肠杆菌K12载体-宿主系统：I. 在lacZ EcoRI限制性位点插入DNA片段。
Mol Gen Genet. 1979 Feb 26;170(2):161-9. doi: 10.1007/BF00337792.
2
Bacteriophage lambda-E. coli K12 vector-host system for gene cloning and expression under lactose promoter control. II. DNA fragment insertion at the vicinity of the lac UV5 promoter.用于在乳糖启动子控制下进行基因克隆和表达的噬菌体λ-大肠杆菌K12载体-宿主系统。II. 在lac UV5启动子附近插入DNA片段。
Mol Gen Genet. 1979 Feb 26;170(2):171-8. doi: 10.1007/BF00337793.
3
The phage promoter responsible for the expression of the inserted beta-galactosidase gene in bacteriophage lambda plac5.负责在噬菌体λplac5中表达插入的β-半乳糖苷酶基因的噬菌体启动子。
Mol Gen Genet. 1980;178(3):555-60. doi: 10.1007/BF00337860.
4
[Variants of phage M13 DNA containing a fragment of the beta-galactosidase gene--a convenient mutation system for the study of oligonucleotide-directed mutagenesis].[含有β-半乳糖苷酶基因片段的噬菌体M13 DNA变体——用于寡核苷酸定向诱变研究的便捷突变系统]
Bioorg Khim. 1986 Dec;12(12):1612-24.
5
Construction and characterization of a tufA-lacZ fusion coding for an E. coli EF-Tu-beta-galactosidase chimeric protein.编码大肠杆菌EF-Tu-β-半乳糖苷酶嵌合蛋白的tufA-lacZ融合基因的构建与特性分析
Mol Gen Genet. 1981;184(2):265-71. doi: 10.1007/BF00272915.
6
Construction of Tn5 lac, a transposon that fuses lacZ expression to exogenous promoters, and its introduction into Myxococcus xanthus.构建将lacZ表达与外源启动子融合的转座子Tn5 lac，并将其导入黄色粘球菌。
Proc Natl Acad Sci U S A. 1984 Sep;81(18):5816-20. doi: 10.1073/pnas.81.18.5816.
7
[Plasmid vectors with a semi-synthetic beta-galactosidase gene of E. coli].[带有大肠杆菌半合成β-半乳糖苷酶基因的质粒载体]
Bioorg Khim. 1983 Sep;9(9):1285-9.
8
Phage lambda and plasmid expression vectors with multiple cloning sites and lacZ alpha-complementation.具有多克隆位点和lacZα互补的噬菌体λ和质粒表达载体。
Gene. 1986;45(1):95-9. doi: 10.1016/0378-1119(86)90136-8.
9
Bacteriophage lambda and plasmid vectors, allowing fusion of cloned genes in each of the three translational phases.λ噬菌体和质粒载体，可使克隆基因在三个翻译阶段中的每个阶段进行融合。
Nucleic Acids Res. 1978 Dec;5(12):4479-94. doi: 10.1093/nar/5.12.4479.
10
Open reading frame cloning: identification, cloning, and expression of open reading frame DNA.开放阅读框克隆：开放阅读框DNA的鉴定、克隆及表达
Proc Natl Acad Sci U S A. 1982 Nov;79(21):6598-602. doi: 10.1073/pnas.79.21.6598.

引用本文的文献

1
Mini-Tn7 Insertion in an Artificial attTn7 Site Enables Depletion of the Essential Master Regulator CtrA in the Phytopathogen Agrobacterium tumefaciens.在人工attTn7位点插入Mini-Tn7可使植物病原菌根癌农杆菌中必需的主调控因子CtrA缺失。
Appl Environ Microbiol. 2016 Jul 29;82(16):5015-25. doi: 10.1128/AEM.01392-16. Print 2016 Aug 15.
2
Highlights of the DNA cutters: a short history of the restriction enzymes.DNA 剪刀的亮点：限制酶的简史。
Nucleic Acids Res. 2014 Jan;42(1):3-19. doi: 10.1093/nar/gkt990. Epub 2013 Oct 18.
3
Broad-host-range expression vectors with tightly regulated promoters and their use to examine the influence of TraR and TraM expression on Ti plasmid quorum sensing.

本文引用的文献

1
Segregation of New Lysogenic Types during Growth of a Doubly Lysogenic Strain Derived from Escherichia Coli K12.源于大肠杆菌K12的双重溶源菌株生长过程中新溶源类型的分离
Genetics. 1954 Jul;39(4):440-52. doi: 10.1093/genetics/39.4.440.
2
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
3
Sensitive mutants of bacteriophage lambda.噬菌体λ的敏感突变体
具有严格调控启动子的广宿主范围表达载体及其用于研究TraR和TraM表达对Ti质粒群体感应的影响。
Appl Environ Microbiol. 2008 Aug;74(16):5053-62. doi: 10.1128/AEM.01098-08. Epub 2008 Jul 7.
4
Cloning of the gene encoding a novel thermostable alpha-galactosidase from Thermus brockianus ITI360.从嗜热栖热菌ITI360中克隆编码一种新型耐热α-半乳糖苷酶的基因。
Appl Environ Microbiol. 1999 Sep;65(9):3955-63. doi: 10.1128/AEM.65.9.3955-3963.1999.
5
Efficient transformation of Dictyostelium discoideum amoebae.盘基网柄菌变形虫的高效转化
Mol Cell Biol. 1983 Dec;3(12):2117-30. doi: 10.1128/mcb.3.12.2117-2130.1983.
6
Characterization of integrated hepatitis B viral DNA cloned from a human hepatoma and the hepatoma-derived cell line PLC/PRF/5.从人肝癌及肝癌衍生细胞系PLC/PRF/5中克隆的整合型乙型肝炎病毒DNA的特性分析
Proc Natl Acad Sci U S A. 1983 May;80(9):2505-9. doi: 10.1073/pnas.80.9.2505.
7
Restriction map of the Escherichia coli malA region and identification of the malT product.大肠杆菌malA区域的限制酶切图谱及malT产物的鉴定
J Bacteriol. 1980 Aug;143(2):761-71. doi: 10.1128/jb.143.2.761-771.1980.
8
Site-specificity of abnormal excision: the mechanism of formation of a specialized transducing bacteriophage lambda plac5.异常切除的位点特异性：特异性转导噬菌体λplac5的形成机制
Nucleic Acids Res. 1984 Sep 11;12(17):6779-95. doi: 10.1093/nar/12.17.6779.
9
Dependence on pH of substrate binding to a mutant lactose carrier, lacYun, in Escherichia coli. A model for H+/lactose symport.大肠杆菌中与突变乳糖载体lacYun结合的底物对pH的依赖性。H⁺/乳糖同向转运模型。
Biochem J. 1989 Mar 1;258(2):389-96. doi: 10.1042/bj2580389.
10
Bacteriophage lambda-E. coli K12 vector-host system for gene cloning and expression under lactose promoter control. II. DNA fragment insertion at the vicinity of the lac UV5 promoter.用于在乳糖启动子控制下进行基因克隆和表达的噬菌体λ-大肠杆菌K12载体-宿主系统。II. 在lac UV5启动子附近插入DNA片段。
Mol Gen Genet. 1979 Feb 26;170(2):171-8. doi: 10.1007/BF00337793.
Virology. 1961 May;14:22-32. doi: 10.1016/0042-6822(61)90128-3.
4
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
5
Studies of novel transducing variants of lambda: dispensability of genes N and Q.λ新型转导变体的研究：基因N和Q的非必需性
Virology. 1969 Oct;39(2):348-52. doi: 10.1016/0042-6822(69)90060-9.
6
The nature of mutants in the lac promoter region.乳糖操纵子启动子区域突变体的性质。
J Mol Biol. 1968 Dec;38(3):421-6. doi: 10.1016/0022-2836(68)90396-3.
7
Direction of transcription of a regulatory gene in E. coli.大肠杆菌中调控基因的转录方向。
Nature. 1968 Dec 28;220(5174):1287-90. doi: 10.1038/2201287a0.
8
Manipulation of restriction targets in phage lambda to form receptor chromosomes for DNA fragments.对噬菌体λ中的限制靶点进行操作，以形成用于DNA片段的受体染色体。
Nature. 1974 Oct 11;251(5475):476-81. doi: 10.1038/251476a0.
9
Properties of a mutant of Escherichia coli defective in bacteriophage lambda head formation (groE). I. Initial characterization.噬菌体λ头部形成缺陷的大肠杆菌突变体（groE）的特性。I. 初步表征。
J Mol Biol. 1973 May 5;76(1):1-23. doi: 10.1016/0022-2836(73)90078-8.
10
Pedigrees of some mutant strains of Escherichia coli K-12.大肠杆菌K-12某些突变菌株的谱系。
Bacteriol Rev. 1972 Dec;36(4):525-57. doi: 10.1128/br.36.4.525-557.1972.