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用于在乳糖启动子控制下进行基因克隆和表达的噬菌体λ-大肠杆菌K12载体-宿主系统。II. 在lac UV5启动子附近插入DNA片段。

Bacteriophage lambda-E. coli K12 vector-host system for gene cloning and expression under lactose promoter control. II. DNA fragment insertion at the vicinity of the lac UV5 promoter.

作者信息

Charnay P, Louise A, Fritsch A, Perrin D, Tiollais P

出版信息

Mol Gen Genet. 1979 Feb 26;170(2):171-8. doi: 10.1007/BF00337793.

DOI:10.1007/BF00337793
PMID:107393
Abstract

Bacteriophage vectors derived from lambda plac5 have been constructed. Their genomes have one EcoRI restriction site which is located at the very beginning of the lac Z gene. The major part of this gene was deleted by an in vivo intramolecular recombination. These vectors allow the fusion of a gene or an operon with the beginning of the lac Z gene, placing them under the control of the lac promoter, which carries the UV5 mutation. Some of these vectors (lambda Y) also include the lac Y gene and it too is under the control of the lac promoter. The lambda YEQS, which carries the Qam73 and Sam7 mutations, as safety mutations, has been certified as a B2 (EK2) vector by the French control commission "recombinaison génétique in vitro".

摘要

已构建出源自λplac5的噬菌体载体。它们的基因组有一个EcoRI限制酶切位点，位于lacZ基因的起始处。该基因的主要部分通过体内分子内重组被删除。这些载体允许一个基因或操纵子与lacZ基因的起始部分融合，使其置于携带UV5突变的lac启动子的控制之下。其中一些载体（λY）还包括lacY基因，它也受lac启动子的控制。携带Qam73和Sam7突变作为安全突变的λYEQS，已被法国“体外基因重组”控制委员会认证为B2（EK2）载体。

相似文献

1
Bacteriophage lambda-E. coli K12 vector-host system for gene cloning and expression under lactose promoter control. II. DNA fragment insertion at the vicinity of the lac UV5 promoter.用于在乳糖启动子控制下进行基因克隆和表达的噬菌体λ-大肠杆菌K12载体-宿主系统。II. 在lac UV5启动子附近插入DNA片段。
Mol Gen Genet. 1979 Feb 26;170(2):171-8. doi: 10.1007/BF00337793.
2
Bacteriophage lambda-E. coli K12 vector-host system for gene cloning and expression under lactose promoter control: I. DNA fragment insertion at the lacZ EcoRI restriction site.用于在乳糖启动子控制下进行基因克隆和表达的噬菌体λ-大肠杆菌K12载体-宿主系统：I. 在lacZ EcoRI限制性位点插入DNA片段。
Mol Gen Genet. 1979 Feb 26;170(2):161-9. doi: 10.1007/BF00337792.
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Bacteriophage lambda and plasmid vectors, allowing fusion of cloned genes in each of the three translational phases.λ噬菌体和质粒载体，可使克隆基因在三个翻译阶段中的每个阶段进行融合。
Nucleic Acids Res. 1978 Dec;5(12):4479-94. doi: 10.1093/nar/5.12.4479.
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Cloning of the lacI gene into A ColE1 plasmid.将lacI基因克隆到一个ColE1质粒中。
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Construction and characterization of the hybrid bacteriophage lambda Charon vectors for DNA cloning.用于DNA克隆的杂交噬菌体λCharon载体的构建与特性分析。
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引用本文的文献

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Appl Environ Microbiol. 2008 Aug;74(16):5053-62. doi: 10.1128/AEM.01098-08. Epub 2008 Jul 7.
2
General method for fine mapping of the Escherichia coli K-12 lamB gene: localization of missense mutations affecting bacteriophage lambda adsorption.大肠杆菌K-12 lamB基因精细定位的通用方法：影响噬菌体λ吸附的错义突变的定位
J Bacteriol. 1981 Dec;148(3):853-60. doi: 10.1128/jb.148.3.853-860.1981.
3

本文引用的文献

1
Segregation of New Lysogenic Types during Growth of a Doubly Lysogenic Strain Derived from Escherichia Coli K12.源于大肠杆菌K12的双重溶源菌株生长过程中新溶源类型的分离
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Sensitive mutants of bacteriophage lambda.噬菌体λ的敏感突变体
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λ受体在大肠杆菌K-12中的合成与成熟：在乳糖启动子控制下基因lamB的体内和体外表达
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Nucleotide sequence for the catalytic domain of colicin E3 and its immunity protein. Evidence for a third gene overlapping colicin.大肠杆菌素E3催化结构域及其免疫蛋白的核苷酸序列。存在第三个与大肠杆菌素重叠基因的证据。
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A lacZ-ftsZ gene fusion is an analog of the cell division inhibitor sulA.lacZ-ftsZ基因融合体是细胞分裂抑制剂sulA的类似物。
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Dependence on pH of substrate binding to a mutant lactose carrier, lacYun, in Escherichia coli. A model for H+/lactose symport.大肠杆菌中与突变乳糖载体lacYun结合的底物对pH的依赖性。H⁺/乳糖同向转运模型。
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7
Cloning in Escherichia coli and physical structure of hepatitis B virion DNA.乙型肝炎病毒粒子DNA在大肠杆菌中的克隆及其物理结构
Proc Natl Acad Sci U S A. 1979 May;76(5):2222-6. doi: 10.1073/pnas.76.5.2222.
8
Bacteriophage lambda-E. coli K12 vector-host system for gene cloning and expression under lactose promoter control: I. DNA fragment insertion at the lacZ EcoRI restriction site.用于在乳糖启动子控制下进行基因克隆和表达的噬菌体λ-大肠杆菌K12载体-宿主系统：I. 在lacZ EcoRI限制性位点插入DNA片段。
Mol Gen Genet. 1979 Feb 26;170(2):161-9. doi: 10.1007/BF00337792.
Virology. 1961 May;14:22-32. doi: 10.1016/0042-6822(61)90128-3.
4
The nature of lactose operator constitive mutations.乳糖操纵基因组成型突变的本质。
J Mol Biol. 1971 Jul 28;59(2):273-305. doi: 10.1016/0022-2836(71)90051-9.
5
Catabolite-insensitive revertants of lac promoter mutants.乳糖启动子突变体的分解代谢物不敏感回复突变体
Proc Natl Acad Sci U S A. 1970 Jul;66(3):773-9. doi: 10.1073/pnas.66.3.773.
6
The nature of mutants in the lac promoter region.乳糖操纵子启动子区域突变体的性质。
J Mol Biol. 1968 Dec;38(3):421-6. doi: 10.1016/0022-2836(68)90396-3.
7
Pedigrees of some mutant strains of Escherichia coli K-12.大肠杆菌K-12某些突变菌株的谱系。
Bacteriol Rev. 1972 Dec;36(4):525-57. doi: 10.1128/br.36.4.525-557.1972.
8
Bacteriophage lambda having EcoRI endonuclease sites only in the nonessential region of the genome.噬菌体λ仅在基因组的非必需区域具有EcoRI核酸内切酶位点。
Proc Natl Acad Sci U S A. 1974 Oct;71(10):3927-30. doi: 10.1073/pnas.71.10.3927.
9
Mechanism of initiation and repression of in vitro transcription of the lac operon of Escherichia coli.大肠杆菌乳糖操纵子体外转录的起始与抑制机制
Proc Natl Acad Sci U S A. 1971 Aug;68(8):1828-32. doi: 10.1073/pnas.68.8.1828.
10
Phage lambda receptor chromosomes for DNA fragments made with restriction endonuclease III of Haemophilus influenzae and restriction endonuclease I of Escherichia coli.用于由流感嗜血杆菌的限制性内切核酸酶III和大肠杆菌的限制性内切核酸酶I制备的DNA片段的噬菌体λ受体染色体。
J Mol Biol. 1975 Nov 5;98(3):551-64. doi: 10.1016/s0022-2836(75)80086-6.