Leonardi R, Villari L, Bernasconi G, Piacentini C, Baciliero U, Travali S
Institute of Orthodontics, University of Catania, Viale A. Doria, Via Biblioteca 4, Catania, Italy.
Arch Oral Biol. 2000 May;45(5):411-8. doi: 10.1016/s0003-9969(99)00144-2.
S-100 protein was detected immunohistochemically in diseased human temporomandibular joint discs with different degrees of pathology, and the findings compared with those of normal discs. In normal discs, large nerve trunks in the posterior ligament were strongly stained by anti-S-100 antiserum; the very few chondrocyte-like cells sometimes showed faint staining, while no staining was observed in any fibrochondrocyte-like or fibroblast-like cell. In dysfunctional discs, S-100 protein immunostaining seemed to correlate with structural pathological findings. The discs showing an abnormal collagen arrangement or fragmentation of collagen fibres presented overall the same immunolabelling pattern as normal discs. In discs with fibrocartilaginous metaplasia and dystrophic cartilage formation, fibrochondrocyte cells showed a very strong immunoreaction for S-100 protein and fibroblast-like cells in some instances were also positive. These findings suggest that S-100 upregulation in disc cells can be considered an attempt at tissue repair by chondroid metaplasia following an injury in that it enables fibroblast-like cells and fibrochondrocytes to acquire a chondrogenic phenotype.
采用免疫组织化学方法检测不同病理程度的人类颞下颌关节盘病变组织中的S-100蛋白，并将结果与正常关节盘进行比较。在正常关节盘中，后韧带中的大神经干被抗S-100抗血清强烈染色；极少数软骨样细胞有时呈淡染色，而在任何纤维软骨样或成纤维细胞样细胞中均未观察到染色。在功能紊乱的关节盘中，S-100蛋白免疫染色似乎与结构病理学结果相关。显示胶原排列异常或胶原纤维断裂的关节盘总体上呈现与正常关节盘相同的免疫标记模式。在伴有纤维软骨化生和营养不良性软骨形成的关节盘中，纤维软骨细胞对S-100蛋白表现出非常强的免疫反应，在某些情况下成纤维细胞样细胞也呈阳性。这些发现表明，关节盘细胞中S-100的上调可被视为损伤后通过类软骨化生进行组织修复的一种尝试，因为它能使成纤维细胞样细胞和纤维软骨细胞获得软骨生成表型。
