Leonardi R, Villari L, Piacentini C, Bernasconi G, Baciliero U, Travali S
Cattedra di Ortognatodonzia, University of Catania, Italy.
Int J Oral Maxillofac Surg. 2000 Aug;29(4):296-300.
The expression pattern of the cell adhesion molecule CD44 standard form (CD44H) in dysfunctional human temporomandibular joint (TMJ) discs was studied immunohistochemically and compared with normal disc pattern in order to evaluate the expression of this adhesion molecule and correlate it to histopathological changes. Immunohistochemistry with anti-CD44H antibodies was performed on paraffin sections of pathological and normal discs. In normal TMJ discs, a moderate immunolabelling with anti-CD44H antibodies was detectable in fibroblastlike cells, in the few fibrochondrocytes and in chondrocytelike cells. In dysfunctional discs, the staining pattern and intensity varied according to the histopathological findings of the specimens. The TMJ discs showing abnormal collagen arrangement or fragmentation of collagen fibres presented overall the same immunolabelling pattern of normal discs. In the discs showing areas of fibrocartilaginous metaplasia, CD44H expression was upregulated in fibrochondrocytes and fibroblastlike cells, especially around the chondroid tissue. Overall, these results suggest that CD44H mediates the binding of some ECM proteins in TMJ disc cells. The up-regulation of CD44H observed in some dysfunctional TMJ discs seems to indicate a prevention of apoptosis in fibroblastlike cells and an important role in phenotypical change of fibrochondrocytes into chondroblastlike cells, enabling the aggregation of chondroid tissue pericellular matrix components.
采用免疫组织化学方法研究细胞黏附分子CD44标准型（CD44H）在功能失调的人类颞下颌关节（TMJ）盘组织中的表达模式，并与正常盘组织模式进行比较，以评估这种黏附分子的表达情况，并将其与组织病理学变化相关联。使用抗CD44H抗体对病理和正常盘组织的石蜡切片进行免疫组织化学检测。在正常TMJ盘中，在成纤维样细胞、少数纤维软骨细胞和软骨样细胞中可检测到抗CD44H抗体的中度免疫染色。在功能失调的盘中，染色模式和强度根据标本的组织病理学结果而有所不同。显示胶原排列异常或胶原纤维断裂的TMJ盘总体上呈现出与正常盘相同的免疫染色模式。在显示纤维软骨化生区域的盘中，CD44H在纤维软骨细胞和成纤维样细胞中表达上调，尤其是在类软骨组织周围。总体而言，这些结果表明CD44H介导TMJ盘细胞中某些细胞外基质蛋白的结合。在一些功能失调的TMJ盘中观察到的CD44H上调似乎表明其可防止成纤维样细胞凋亡，并在纤维软骨细胞向成骨细胞样细胞的表型变化中起重要作用，从而使类软骨组织细胞周围基质成分聚集。
