人核苷二磷酸激酶A的结晶及初步X射线晶体学分析
Crystallization and preliminary X-ray crystallographic analysis of human nucleoside diphosphate kinase A.
作者信息
Min K, Kim S Y, Song H K, Chang C, Cho S J, Moon J, Yang J K, Lee J Y, Lee K J, Suh S W
机构信息
Department of Chemistry, College of Natural Sciences, Seoul National University, Seoul 151--742, South Korea.
出版信息
Acta Crystallogr D Biol Crystallogr. 2000 Apr;56(Pt 4):503-4.
Human nucleoside diphosphate kinase A catalyzes phosphoryl transfer and acts as a suppressor of metastasis. It has been crystallized using 2-methyl-2,4-pentanediol as a precipitant at 288 K. The crystal is monoclinic, belonging to the space group P2(1), with unit-cell parameters a = 74.21, b = 78.11, c = 82.29 A, beta = 101. 33 degrees. The asymmetric unit contains a homohexamer, with a corresponding crystal volume per protein mass (V(m)) of 2.27 A(3) Da(-1) and a solvent content of 46%. Native X-ray data to 2.15 A resolution have been collected using synchrotron X-rays.
人核苷二磷酸激酶A催化磷酸转移反应，并作为转移抑制因子发挥作用。它已在288 K下使用2-甲基-2,4-戊二醇作为沉淀剂进行结晶。晶体为单斜晶系，属于空间群P2(1)，晶胞参数a = 74.21、b = 78.11、c = 82.29 Å，β = 101.33°。不对称单元包含一个同六聚体，每个蛋白质质量对应的晶体体积（V(m)）为2.27 ų Da⁻¹，溶剂含量为46%。已使用同步加速器X射线收集了分辨率达2.15 Å的天然X射线数据。
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