Li L, Garden R W, Sweedler J V
Department of Chemistry and Beckman Institute, University of Illinois, Urbana, IL 61801, USA.
Trends Biotechnol. 2000 Apr;18(4):151-60. doi: 10.1016/s0167-7799(00)01427-x.
Matrix-assisted laser desorption-ionization (MALDI) mass spectrometry (MS) is a rapid and sensitive analytical approach that is well suited for obtaining molecular weights of peptides and proteins from complex samples. MALDI-MS can profile the peptides and proteins from single-cell and small tissue samples without the need for extensive sample preparation, except for the cell isolation and matrix application. Strategies for peptide identification and characterization of post-translational modifications are presented. Furthermore, several recent enhancements in MALDI-MS technology, including in situ peptide sequencing as well as the direct spatial mapping of peptides in cells and tissues are discussed.
基质辅助激光解吸电离（MALDI）质谱（MS）是一种快速且灵敏的分析方法，非常适合从复杂样品中获取肽和蛋白质的分子量。除了细胞分离和基质应用外，MALDI-MS无需进行大量样品制备，就能对单细胞和小组织样品中的肽和蛋白质进行分析。本文介绍了肽鉴定策略以及翻译后修饰的表征方法。此外，还讨论了MALDI-MS技术最近的一些改进，包括原位肽测序以及细胞和组织中肽的直接空间定位。
