Chen Y, Song J
Key Laboratory of Cell Biology, Ministry of Public Health of China, China Medical University, Shenyang.
Zhonghua Zhong Liu Za Zhi. 1997 Mar;19(2):81-4.
To study the biological significance of Grp94 deleted product (Grp94 beta) expressed in human colorectal carcinoma cells.
The relationship between molecular chaperone Grp94 and c-myc oncogene expression in the human colorectal carcinoma cell line CCL229, CX-1 and retinoic acid (RA) induced CCL229 by both of digoxin labelled c-myc cDNA probe spot by-bridization and RT-PCR with endoplasmic reticulum molecular chaperone Grp94 proximal 3' end oligonucletide primers was investigated.
In CCL229 cells, the expression of Grp94 beta and c-myc oncogene are significantly higher than those in CX-1 cells. Along with the RA inducing, both decreased with one accord, and to the lowest level after six days induction.
The Grp94 normal expression (Grp94 alpha), which was undetectable in CCL229 cells increased after RA induction. The results showed that the expression of Grp94 beta may be closely related to that of c-myc oncogene. It is suggested that the Grp94 be related to some biological characteristics of cancer, for example, the invasiveness.
研究人结肠癌细胞中表达的葡萄糖调节蛋白94缺失产物（Grp94β）的生物学意义。
采用地高辛标记的c-myc cDNA探针斑点杂交和用内质网分子伴侣Grp94近端3'端寡核苷酸引物进行的RT-PCR，研究分子伴侣Grp94与人结肠癌细胞系CCL229、CX-1以及维甲酸（RA）诱导的CCL229中c-myc癌基因表达之间的关系。
在CCL229细胞中，Grp94β和c-myc癌基因的表达明显高于CX-1细胞。随着RA诱导，二者均一致下降，诱导6天后降至最低水平。
在CCL229细胞中未检测到的Grp94正常表达（Grp94α）在RA诱导后增加。结果表明，Grp94β的表达可能与c-myc癌基因的表达密切相关。提示Grp94与癌症的某些生物学特性有关，例如侵袭性。
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