Cox S T, McWhinnie A, Prokupek B, Madrigal J A, Little A M
Anthony Nolan Research Institute, The Royal Free Hospital, London, UK.
Tissue Antigens. 2000 Feb;55(2):185-7. doi: 10.1034/j.1399-0039.2000.550215.x.
We describe a novel allele belonging to the B44 allele group. HLA-B4413 was identified in a Caucasoid potential bone marrow donor from the Anthony Nolan Bone Marrow Trust register. Initial HLA typing of this donor revealed unusual serological reactivity. Further investigation was carried out by reference strand conformation analysis (RSCA) and sequence-based typing (SBT) using DNA extracted from an Epstein-Barr virus (EBV)-transformed B-cell line made from this donor (AMI005AN). In this individual, two B44 alleles were revealed upon initial investigation: B4409 and a previously unseen allele which has been named B4413. B4413 is identical to B*44031 except for a unique nucleotide substitution resulting in an amino acid difference at residue 61 in the alpha1 helix.
我们描述了一个属于B44等位基因组的新等位基因。HLA-B4413是在安东尼·诺兰骨髓信托登记处的一名白种人潜在骨髓供者中鉴定出来的。该供者最初的HLA分型显示出异常的血清学反应性。通过参考链构象分析（RSCA）和基于序列的分型（SBT），使用从该供者（AMI005AN）建立的爱泼斯坦-巴尔病毒（EBV）转化B细胞系中提取的DNA进行了进一步研究。在这名个体中，初步研究发现了两个B44等位基因：B4409和一个以前未见过的等位基因，已将其命名为B4413。B4413与B*44031相同，只是有一个独特的核苷酸取代，导致α1螺旋中第61位氨基酸存在差异。
