Binzoni T, Hiltbrand E, Terrier F, Cerretelli P, Delpy D
Depts. of Radiology and Physiology, Faculty of Medicine, University of Geneva, Geneva, Switzerland.
Magn Reson Med. 2000 Apr;43(4):611-4. doi: 10.1002/(sici)1522-2594(200004)43:4<611::aid-mrm18>3.0.co;2-i.
It is well established that ADP is an important regulator of the oxidative phosphorylation in the mitochondria. Thus, by means of noninvasive techniques it is demonstrated that the relationship between O(2) consumption of the human gastrocnemius at rest and its temperature is likely determined by at least two factors: 1) the modulation of the rate of the chemical reactions imposed by the "physical" temperature-effect; 2) the influence of temperature-induced ADP concentration changes ( approximately 0.83 microM degrees C(-1)) on oxidative phosphorylation. ADP was assessed by applying the temperature-corrected Lohmann equilibrium equation. PCr and ATP were found to increase, with decreasing temperature (-0.54+/-0.05 and -0.17+/-mM degrees C(-1), respectively), while pH varies following the alpha-stat hypothesis (-0.016+/-0.001 pH degrees C(-1)). These findings should be of value when dealing with muscle physiology in extreme environments or clinical applications of hypothermia.
众所周知，二磷酸腺苷（ADP）是线粒体氧化磷酸化的重要调节因子。因此，通过非侵入性技术表明，人体腓肠肌静息时的耗氧量与其温度之间的关系可能至少由两个因素决定：1）“物理”温度效应所施加的化学反应速率的调节；2）温度诱导的ADP浓度变化（约0.83微摩尔/摄氏度）对氧化磷酸化的影响。通过应用温度校正的洛曼平衡方程来评估ADP。发现磷酸肌酸（PCr）和三磷酸腺苷（ATP）随温度降低而增加（分别为-0.54±0.05和-0.17±毫摩尔/摄氏度），而pH值则遵循α-稳态假说变化（-0.016±0.001pH/摄氏度）。这些发现对于在极端环境中处理肌肉生理学或低温的临床应用时具有重要价值。
