Reubinoff B E, Pera M F, Fong C Y, Trounson A, Bongso A
Monash Institute of Reproduction & Development, Monash University, Melbourne, Victoria, Australia.
Nat Biotechnol. 2000 Apr;18(4):399-404. doi: 10.1038/74447.
We describe the derivation of pluripotent embryonic stem (ES) cells from human blastocysts. Two diploid ES cell lines have been cultivated in vitro for extended periods while maintaining expression of markers characteristic of pluripotent primate cells. Human ES cells express the transcription factor Oct-4, essential for development of pluripotential cells in the mouse. When grafted into SCID mice, both lines give rise to teratomas containing derivatives of all three embryonic germ layers. Both cell lines differentiate in vitro into extraembryonic and somatic cell lineages. Neural progenitor cells may be isolated from differentiating ES cell cultures and induced to form mature neurons. Embryonic stem cells provide a model to study early human embryology, an investigational tool for discovery of novel growth factors and medicines, and a potential source of cells for use in transplantation therapy.
我们描述了从人类囊胚中获取多能胚胎干细胞（ES细胞）的过程。已在体外长期培养了两条二倍体ES细胞系，同时维持着多能灵长类细胞特征性标志物的表达。人类ES细胞表达转录因子Oct-4，这对小鼠多能细胞的发育至关重要。当移植到SCID小鼠体内时，这两条细胞系都会产生含有所有三个胚胎胚层衍生物的畸胎瘤。这两条细胞系在体外均可分化为胚外和体细胞谱系。神经祖细胞可从分化的ES细胞培养物中分离出来，并诱导形成成熟神经元。胚胎干细胞为研究早期人类胚胎学提供了一个模型，是发现新型生长因子和药物的研究工具，也是移植治疗中潜在的细胞来源。
