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[某些氨基酸对培养的内孢霉属菌种合成糖化酶的影响]

[Effect of some amino acids on the biosynthesis of glucoamylase by cultured Endomycopsis species].

作者信息

Gracheva I M, Gernet M V, Gavrilova N N, Razarenova N F

出版信息

Prikl Biokhim Mikrobiol. 1978 Nov-Dec;14(6):871-7.

PMID:107516
Abstract

The effect of amino acids (glutamic, aspartic, beta-alanine, phenylalanine, methionine) on the glucoamylase biosynthesis by Endomycopsis species 20-9 was studied. Monoaminodicarboxylic acids produced a stimulating effect on the glucoamylase synthesis. Their addition at optimal concentrations of 0.2 to 0.4% increased by 54--69% glucoamylase activity of Endomycopsis sp. 20-9 upon submerged cultivation on the medium containing 3% maize extract and 1% starch. An application of this amino acid nutrition during cultivation did not produce a significant effect on the microbial glucoamylase activity.

摘要

研究了氨基酸(谷氨酸、天冬氨酸、β-丙氨酸、苯丙氨酸、甲硫氨酸)对20-9种内孢霉属菌株合成糖化酶的影响。单氨基二羧酸对糖化酶的合成有刺激作用。在含有3%玉米提取物和1%淀粉的培养基上进行深层培养时,以0.2%至0.4%的最佳浓度添加这些氨基酸,可使20-9种内孢霉属菌株的糖化酶活性提高54%至69%。在培养过程中应用这种氨基酸营养对微生物糖化酶活性没有显著影响。

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