Lundby L, Overgaard J, Laurberg S
Department of Surgery, Aarhus University Hospital, Denmark.
APMIS. 2000 Mar;108(3):216-22. doi: 10.1034/j.1600-0463.2000.d01-47.x.
The aim of the present study was to assess and quantify changes in the structural components of the rectal wall after irradiation with varying single doses of x-rays. A total of 70 CDF1/Bom male mice were irradiated at a selective 1.5 cm of the distal rectum with varying single doses of 0-30 Gy. At 32 weeks postirradiation the mice were sacrificed and the rectum was removed. Sampling of the specimens was based on unbiased stereological principles using systematic random sampling. Vertical tissue sections were used to estimate mucosal surface area density and to measure the thickness and volume fraction of the different intestinal wall layers. The surface area density decreased with increasing dose (p<0.02) due to pronounced injury of the crypts and mucosal morphology. The thickness and the volume fraction of the submucosa were significantly increased in the 20, 25 and 30 Gy treatment groups (p<0.0001 and p<0.0001), whereas the thicknesses of the mucosa, the lamina muscularis propria and the serosa did not change after irradiation. It is concluded that irradiation causes histopathological changes in the rectal mucosa and submucosa, and changes in the thickness of the submucosal intestinal layer. These changes were found to be dose-dependent.
本研究的目的是评估和量化不同单次剂量X射线照射后直肠壁结构成分的变化。总共70只CDF1/Bom雄性小鼠在距直肠远端1.5厘米处接受0 - 30 Gy不同单次剂量的照射。照射后32周处死小鼠并取出直肠。标本采样基于无偏立体学原理，采用系统随机抽样。垂直组织切片用于估计黏膜表面积密度，并测量不同肠壁层的厚度和体积分数。由于隐窝和黏膜形态的明显损伤，表面积密度随剂量增加而降低（p<0.02）。在20、25和30 Gy治疗组中，黏膜下层的厚度和体积分数显著增加（p<0.0001和p<0.0001），而照射后黏膜、固有肌层和浆膜的厚度没有变化。结论是，照射会导致直肠黏膜和黏膜下层的组织病理学变化，以及黏膜下肠层厚度的变化。发现这些变化是剂量依赖性的。
