Sousa Marcos Vieira de, Priolli Denise Gonçalves, Portes Adriana Valim, Cardinalli Izilda Aparecida, Pereira José Aires, Martinez Carlos Augusto Real
USF, Bragança Paulista, Sao Paulo, Brazil.
Acta Cir Bras. 2008 Sep-Oct;23(5):417-24. doi: 10.1590/s0102-86502008000500005.
To evaluate histopathological alterations of the colon wall in segments with and without intestinal transit, by computer-assisted imaging, and to correlate these with the length of time diversion.
Thirty male Wistar rats were subjected to intestinal transit diversion by a proximal colostomy and distal mucosa fistula. The animals were divided into three experimental groups according to how long after the initial surgical procedure they were sacrificed: six, twelve and eighteen weeks. Colon segments with and without transit were subjected to histopathological study. The variables colon crypt length, mucosal ulceration, muscle layer thickness of the muscularis mucosa, submucosa and muscularis propria, vascular congestion, number of caliciform cells, inflammatory grade and degree of inflammation, comparing the two colon segments in the different experimental groups were studied. Intestinal crypt length, muscle layer thickness of the mucosa, submucosa and muscularis propria and caliciform cells were measured by computer-assisted imaging method. Mean equality, variance analysis and correlation tests were used in the statistical analysis, and the significance level was set at 5%.
Comparison between segments with and without transit showed that the latter presented reduced length of colon crypts and increased muscle layer thickness of the muscularis mucosa, submucosa and muscularis propria. There were greater quantities of ulceration of the mucosal and greater degree of inflammation with increasing time without transit. Mucosal ulceration, submucosal vascular congestion, increased thickness of the submucosal and muscularis propria layers, presence of caliciform cells, inflammatory infiltrate and inflammatory grade correlated significantly with the length of time without transit.
Histological alterations occurred in all layers of the colon wall, in the segments without intestinal transit. Ulcerations in the intestinal mucosa, increased number of caliciform cells, greater vascular congestion of the submucosal layer and inflammatory reaction were related to increasing length of time without transit.
通过计算机辅助成像评估有和无肠转运的结肠段肠壁的组织病理学改变，并将这些改变与肠转流时间相关联。
30只雄性Wistar大鼠通过近端结肠造口术和远端黏膜瘘进行肠转流。根据初次手术后处死动物的时间将其分为三个实验组：6周、12周和18周。对有和无肠转运的结肠段进行组织病理学研究。研究不同实验组中两个结肠段的结肠隐窝长度、黏膜溃疡、黏膜肌层、黏膜下层和固有肌层的肌层厚度、血管充血、杯状细胞数量、炎症分级和炎症程度等变量。通过计算机辅助成像方法测量肠隐窝长度、黏膜、黏膜下层和固有肌层的肌层厚度以及杯状细胞。统计分析采用均值相等性检验、方差分析和相关性检验，显著性水平设定为5%。
有和无肠转运的结肠段之间的比较显示，无肠转运的结肠段结肠隐窝长度缩短，黏膜肌层、黏膜下层和固有肌层的肌层厚度增加。随着无肠转运时间的增加，黏膜溃疡数量更多，炎症程度更高。黏膜溃疡、黏膜下层血管充血、黏膜下层和固有肌层厚度增加、杯状细胞的存在、炎症浸润和炎症分级与无肠转运时间显著相关。
在无肠转运的结肠段，结肠壁的所有层均发生了组织学改变。肠黏膜溃疡、杯状细胞数量增加、黏膜下层血管充血加剧以及炎症反应与无肠转运时间的增加有关。
