编码小鼠双微体2（mdm2）原癌基因犬类和马类同源物的cDNA的克隆、序列分析及表达

Cloning, sequence analysis and expression of the cDNAs encoding the canine and equine homologues of the mouse double minute 2 (mdm2) proto-oncogene.

作者信息

Nasir L, Burr P D, McFarlane S T, Gault E, Thompson H, Argyle D J

机构信息

Molecular Therapeutics Research Group, Department of Veterinary Clinical Studies, University of Glasgow Veterinary School, Bearsden Road, Glasgow, UK.

出版信息

Cancer Lett. 2000 Apr 28;152(1):9-13. doi: 10.1016/s0304-3835(99)00427-9.

DOI:10.1016/s0304-3835(99)00427-9

PMID:10754200

Abstract

The mdm2 oncogene is amplified and overexpressed in a variety of human tumours and the oncogenic potential of MDM2 is partly due to its ability to inactivate tumour suppressor p53 function. In the present communication we describe the cloning, sequence analysis and expression of the complete wildtype canine and equine mdm2 cDNAs. The encoded full-length canine and equine cDNAs show strong sequence homology with MDM2 proteins from other species and both cDNAs generate recombinant proteins of approximately 90 kDa. These data will allow for the role of this oncogene to be established in companion animal oncology.

摘要

mdm2癌基因在多种人类肿瘤中发生扩增并过度表达，MDM2的致癌潜力部分归因于其使肿瘤抑制因子p53功能失活的能力。在本通讯中，我们描述了完整野生型犬和马mdm2 cDNA的克隆、序列分析及表达。编码的全长犬和马cDNA与其他物种的MDM2蛋白具有很强的序列同源性，并且这两种cDNA均产生约90 kDa的重组蛋白。这些数据将有助于确定该癌基因在伴侣动物肿瘤学中的作用。