Jones S N, Ansari-Lari M A, Hancock A R, Jones W J, Gibbs R A, Donehower L A, Bradley A
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA.
Gene. 1996 Oct 10;175(1-2):209-13. doi: 10.1016/0378-1119(96)00151-5.
Transfection of the mouse double minute 2 (Mdm2) oncogene has been found to induce immortalization of primary cells and to transform cultured cells. Amplification and/or overexpression of human MDM2 has been documented in a large percentage of human cancers. Mouse and human Mdm2 cDNA have been cloned from transformed cells and the cDNA sequence of both genes have been reported previously. In this report, we present the gene structure of mouse Mdm2. Comparison of the coding sequences of the Mdm2 gene with the previously reported cDNA sequence and with Mdm2 sequences obtained from an Mdm2-bearing cosmid clone capable of inducing transformation revealed that the reported cDNA sequence was in error, and that Mdm2-induced transformation of cells does not require an activating mutation in Mdm2. Ligation-anchor PCR analysis of transcripts produced from the P1 and P2 promoters indicates that transcription initiates at sites upstream of those reported previously for both promoters.
已发现转染小鼠双微体2（Mdm2）癌基因可诱导原代细胞永生化并转化培养细胞。在很大比例的人类癌症中已记录到人类MDM2的扩增和/或过表达。小鼠和人类Mdm2 cDNA已从转化细胞中克隆出来，并且这两个基因的cDNA序列先前已被报道。在本报告中，我们展示了小鼠Mdm2的基因结构。将Mdm2基因的编码序列与先前报道的cDNA序列以及从能够诱导转化的携带Mdm2的黏粒克隆获得的Mdm2序列进行比较，结果表明报道的cDNA序列有误，并且Mdm2诱导的细胞转化并不需要Mdm2中的激活突变。对由P1和P2启动子产生的转录本进行连接锚定PCR分析表明，转录起始于先前报道的两个启动子上游的位点。
