Yokota S, Amano K, Fujii N, Yokochi T
Central Research Laboratory, Akita University School of Medicine, 1-1-1, Hondo, Akita, Japan.
FEMS Microbiol Lett. 2000 Apr 15;185(2):193-8. doi: 10.1111/j.1574-6968.2000.tb09061.x.
We examined the levels of antibody titers against Helicobacter pylori antigens, three types of lipopolysaccharides (LPSs), recombinant CagA antigen, recombinant VacA antigen and partially purified cellular antigens in the sera of Japanese volunteers. The three types of LPSs are LPS carrying the highly antigenic epitope, LPS carrying the weakly antigenic epitope and rough LPS, classified on the basis of antigenicity in humans. IgG titers against all H. pylori antigens tested were significantly different between gastroduodenal patients and healthy adults without H. pylori infection. IgG titers against LPS carrying the weakly antigenic epitope, rough LPS and VacA antigen, as well as IgA titers against the partially purified cellular extract were significantly higher in gastroduodenal patients than in H. pylori-positive healthy adults. However, IgG titers against LPS carrying the highly antigenic epitope, CagA antigen or the partially purified cellular extract showed no significant difference between patients and H. pylori-positive healthy adults. The results indicated that increases in IgG titers against VacA antigen and the weakly antigenic and core epitopes of LPS, and in IgA titer against the partially purified cellular extract, were associated with disease state and may be useful in identifying active infection of H. pylori.
我们检测了日本志愿者血清中针对幽门螺杆菌抗原、三种脂多糖（LPS）、重组CagA抗原、重组VacA抗原及部分纯化细胞抗原的抗体滴度水平。这三种LPS分别是携带高抗原性表位的LPS、携带弱抗原性表位的LPS和粗糙型LPS，是根据其对人类的抗原性进行分类的。在胃十二指肠疾病患者和未感染幽门螺杆菌的健康成年人之间，针对所有检测的幽门螺杆菌抗原的IgG滴度存在显著差异。胃十二指肠疾病患者针对携带弱抗原性表位的LPS、粗糙型LPS和VacA抗原的IgG滴度，以及针对部分纯化细胞提取物的IgA滴度，均显著高于幽门螺杆菌阳性的健康成年人。然而，针对携带高抗原性表位的LPS、CagA抗原或部分纯化细胞提取物的IgG滴度，在患者和幽门螺杆菌阳性的健康成年人之间无显著差异。结果表明，针对VacA抗原、LPS的弱抗原性和核心表位的IgG滴度升高，以及针对部分纯化细胞提取物的IgA滴度升高，与疾病状态相关，可能有助于识别幽门螺杆菌的活动性感染。
