Yokota S i, Amano K i, Hayashi S, Kubota T, Fujii N, Yokochi T
Sumitomo Pharmaceuticals Research Center, Konohana-ku, Osaka 554, Japan.
Infect Immun. 1998 Jun;66(6):3006-11. doi: 10.1128/IAI.66.6.3006-3011.1998.
We have examined the antibody response to Helicobacter pylori lipopolysaccharides (LPS) in humans. We used sera from patients with gastroduodenal diseases and healthy adults infected or not infected with H. pylori. Data from the experiments for antibody binding to LPS suggested that the polysaccharide chains from many H. pylori strains showed high immunogenicity in humans. Sera from most (above 70%) H. pylori-infected individuals contained immunoglobulin G (IgG) antibodies against the polysaccharide region highly immunogenic H. pylori LPS. The IgG titers of individual serum samples that reacted strongly with highly immunogenic LPS were quite similar (r2 = 0.84 to 0.98). The results suggest wide distribution among H. pylori strains of a highly antigenic epitope in the polysaccharide moieties of their LPS. Also, the similarity in the titers of individual serum samples against highly immunogenic LPS points to the existence of epitopes sharing a common structural motif. However, some strains showed low antigenicity, even those with polysaccharide-carrying LPS. The dominant subclass of IgG that reacted with the highly immunogenic LPS was IgG2, which was preferentially raised against polysaccharide antigens. Recently, a structure that mimics that of the Lewis antigens was identified in the O-polysaccharide fraction of H. pylori LPS; however, no correlation between antigenicity of the polysaccharide chain in humans and the presence of Lewis antigens was found. The IgA and IgM titers against H. pylori LPS seemed to be mostly nonspecific and directed against lipid A. In a few cases, however, sera from individuals infected with H. pylori gave strong IgA and IgM titers against the highly immunogenic polysaccharide. In conclusion, the LPS of many H. pylori strains possess an antigenic epitope in their polysaccharide regions that is immunogenic in humans. However, our results show that the antigenic epitope is unlikely to be immunologically related to structures mimicking Lewis antigens.
我们检测了人类对幽门螺杆菌脂多糖（LPS）的抗体反应。我们使用了患有胃十二指肠疾病的患者以及感染或未感染幽门螺杆菌的健康成年人的血清。抗体与LPS结合实验的数据表明，许多幽门螺杆菌菌株的多糖链在人类中显示出高免疫原性。大多数（超过70%）感染幽门螺杆菌的个体血清中含有针对高免疫原性幽门螺杆菌LPS多糖区域的免疫球蛋白G（IgG）抗体。与高免疫原性LPS强烈反应的个体血清样本的IgG滴度非常相似（r2 = 0.84至0.98）。结果表明，幽门螺杆菌菌株中其LPS多糖部分存在高度抗原性表位的广泛分布。此外，个体血清样本针对高免疫原性LPS的滴度相似性表明存在具有共同结构基序的表位。然而，一些菌株显示出低抗原性，即使是那些带有多糖的LPS菌株。与高免疫原性LPS反应的IgG主要亚类是IgG2，它优先针对多糖抗原产生。最近，在幽门螺杆菌LPS的O-多糖部分中鉴定出一种模拟Lewis抗原的结构；然而，未发现人类中多糖链的抗原性与Lewis抗原的存在之间存在相关性。针对幽门螺杆菌LPS的IgA和IgM滴度似乎大多是非特异性的，且针对脂质A。然而，在少数情况下，感染幽门螺杆菌个体的血清对高免疫原性多糖产生了强烈的IgA和IgM滴度。总之，许多幽门螺杆菌菌株的LPS在其多糖区域具有在人类中具有免疫原性的抗原表位。然而，我们的结果表明，该抗原表位不太可能与模拟Lewis抗原的结构存在免疫相关性。
