Seo Ji-Hyun, Lim Chun Woo, Park Ji Sook, Yeom Jung Sook, Lim Jae-Young, Jun Jin-Su, Woo Hyang-Ok, Youn Hee-Shang, Baik Seung-Chul, Lee Woo-Kon, Cho Myung-Je, Rhee Kwang-Ho
Department of Pediatrics, Gyeongsang National University School of Medicine, Gyeongsang Institute of Health Science, Jinju, Korea .
Department of Microbiology, Gyeongsang National University School of Medicine, Gyeongsang Institute of Health Science, Jinju, Korea .
J Korean Med Sci. 2016 Mar;31(3):417-22. doi: 10.3346/jkms.2016.31.3.417. Epub 2016 Feb 17.
We tested correlations between anti-Helicobacter pylori IgG and IgA levels and the urease test, anti-CagA protein antibody, degree of gastritis, and age. In total, 509 children (0-15 years) were enrolled. Subjects were stratified as 0-4 years (n = 132), 5-9 years (n = 274), and 10-15 years (n = 103) and subjected to the urease test, histopathology, ELISA, and western blot using whole-cell lysates of H. pylori strain 51. The positivity rate in the urease test (P = 0.003), the degree of chronic gastritis (P = 0.021), and H. pylori infiltration (P < 0.001) increased with age. The median titer for anti-H. pylori IgG was 732.5 IU/mL at 0-4 years, 689.0 IU/mL at 5-9 years, and 966.0 IU/mL at 10-15 years (P < 0.001); the median titer for anti-H. pylori IgA was 61.0 IU/mL at 0-4 years, 63.5 IU/mL at 5-9 years, and 75.0 IU/mL at 10-15 years (P < 0.001). The CagA-positivity rate was 26.5% at 0-4 years, 36.5% at 5-9 years, and 46.6% at 10-15 years for IgG (P = 0.036), and 11.3% at 0-4 years, 18.6% at 5-9 years, and 23.3% at 10-15 years for IgA (P < 0.001). Anti-H. pylori IgG and IgA titers increased with the urease test grade, chronic gastritis degree, active gastritis, and H. pylori infiltration. Presence of CagA-positivity is well correlated with a high urease test grade and high anti-H. pylori IgG/IgA levels.
我们检测了抗幽门螺杆菌IgG和IgA水平与尿素酶试验、抗CagA蛋白抗体、胃炎程度及年龄之间的相关性。总共纳入了509名儿童（0至15岁）。将受试者分为0至4岁组（n = 132）、5至9岁组（n = 274）和10至15岁组（n = 103），并对其进行尿素酶试验、组织病理学检查、酶联免疫吸附测定（ELISA）以及使用幽门螺杆菌51菌株的全细胞裂解物进行蛋白质印迹法检测。尿素酶试验的阳性率（P = 0.003）、慢性胃炎程度（P = 0.021）和幽门螺杆菌浸润程度（P < 0.001）均随年龄增长而升高。0至4岁组抗幽门螺杆菌IgG的中位滴度为732.5 IU/mL，5至9岁组为689.0 IU/mL，10至15岁组为966.0 IU/mL（P < 0.001）；0至4岁组抗幽门螺杆菌IgA的中位滴度为61.0 IU/mL，5至9岁组为63.5 IU/mL，10至15岁组为75.0 IU/mL（P < 0.001）。IgG的CagA阳性率在0至4岁组为26.5%，5至9岁组为36.5%，10至15岁组为46.6%（P = 0.036）；IgA的CagA阳性率在0至4岁组为11.3%，5至9岁组为18.6%，10至15岁组为23.3%（P < 0.001）。抗幽门螺杆菌IgG和IgA滴度随尿素酶试验分级、慢性胃炎程度、活动性胃炎及幽门螺杆菌浸润程度的增加而升高。CagA阳性与高尿素酶试验分级及高抗幽门螺杆菌IgG/IgA水平密切相关。
