Tanaka K, Otsuki T, Sonoo H, Yamamoto Y, Udagawa K, Kunisue H, Arime I, Yamamoto S, Kurebayashi J, Shimozuma K
Department of Breast and Thyroid Surgery, Kawasaki Medical School, Kurashiki, 701-0192, Japan.
Eur J Endocrinol. 2000 Apr;142(4):340-6. doi: 10.1530/eje.0.1420340.
To investigate the levels of expression of the sodium iodide symporter (NIS) and three differentiation markers (thyroglobulin (Tg), thyroid peroxidase (TPO) and thyrotrophin receptor (TSH-R)) in 35 patients with primary (n=31) or recurrent (n=4) papillary thyroid carcinoma, and to compare the findings with clinical data.
We performed a multiplex semi-quantitative RT-PCR to analyse the relative levels of expression of Tg, TPO and TSH-R mRNAs, and a separate semi-quantitative RT-PCR for NIS mRNA.
Tg, TPO and TSH-R mRNAs were expressed in all the patients, whereas NIS mRNA was expressed in all but eight. Analysis of the expression of the differentiation markers in all patients showed a significant correlation among Tg, TPO and NIS. With regard to the relationship between the expression of each gene and the MACIS score, there was significant correlation only for the Tg gene (P<0.05).
The levels of expression of NIS mRNA correlated significantly with those of Tg and TPO mRNAs, but not with those of TSH-R mRNA. The relationship with clinical stage and prognostic score, however, varied among these differentiation markers.
研究35例原发性（n = 31）或复发性（n = 4）甲状腺乳头状癌患者中碘化钠转运体（NIS）及三种分化标志物（甲状腺球蛋白（Tg）、甲状腺过氧化物酶（TPO）和促甲状腺激素受体（TSH-R））的表达水平，并将结果与临床数据进行比较。
我们进行了多重半定量逆转录聚合酶链反应（RT-PCR），以分析Tg、TPO和TSH-R mRNA的相对表达水平，并对NIS mRNA进行单独的半定量RT-PCR。
所有患者均表达Tg、TPO和TSH-R mRNA，而除8例患者外，其余患者均表达NIS mRNA。对所有患者分化标志物表达的分析显示，Tg、TPO和NIS之间存在显著相关性。关于每个基因的表达与MACIS评分之间的关系，仅Tg基因存在显著相关性（P < 0.05）。
NIS mRNA的表达水平与Tg和TPO mRNA的表达水平显著相关，但与TSH-R mRNA的表达水平无关。然而，这些分化标志物与临床分期和预后评分的关系各不相同。
