Nakayama Y, Shirai Y, Yoshihara K, Uesaka S
Department of Orthopaedic Surgery, Nippon Medical School, Tokyo, Japan.
J Nippon Med Sch. 2000 Apr;67(2):92-5. doi: 10.1272/jnms.67.92.
Glycosaminoglycans (GAGs) level in joint fluid have been investigated in various joint diseases as a joint maker. However, there are very few studies of normal joint fluid providing a baseline for a better understanding of altered GAGs level in pathological joint fluid. We investigated GAGs level in knee joint fluid for 25 healthy young volunteers with a mean age of 27.5 years (range: 18 to 36 years). Biochemical evaluations included the concentration of hyaluronic acid (HA), chondroitin 4-sulfate (C4S) and chondroitin 6-sulfate (C6S), and the C6S/C4S ratio. The unsaturated disaccharides derived from HA by Morgan-Elson methods, and those from CS were measured with high performance liquid chromatography. The mean HA concentration was 3.4+/-0.6 mg/ml. The mean concentration of C4S and C6S, and C6S/C4S ratio were 19.0+/-4.9 nmol/ml, 125.5+/-44.4 nmol/ml and 6.5+/-1.1 respectively.
作为一种关节标志物，人们已对各种关节疾病中关节液中的糖胺聚糖（GAGs）水平进行了研究。然而，关于正常关节液的研究非常少，这些研究可为更好地理解病理性关节液中GAGs水平的变化提供基线。我们对25名平均年龄为27.5岁（范围：18至36岁）的健康年轻志愿者的膝关节液中的GAGs水平进行了研究。生化评估包括透明质酸（HA）、硫酸软骨素4（C4S）和硫酸软骨素6（C6S）的浓度，以及C6S/C4S比值。通过摩根-埃尔森方法从HA衍生的不饱和二糖，以及从CS衍生的不饱和二糖，用高效液相色谱法进行测定。HA的平均浓度为3.4±0.6毫克/毫升。C4S和C6S的平均浓度以及C6S/C4S比值分别为19.0±4.9纳摩尔/毫升、125.5±44.4纳摩尔/毫升和6.5±1.1。
