Prakasham R S, Kuriakose B, Ramakrishna S V
Department of Biochemical and Environmental Engineering, Indian Institute of Chemical Technology, Hyderabad, India.
Appl Biochem Biotechnol. 1999 Nov;82(2):127-34. doi: 10.1385/abab:82:2:127.
The catalytic role of various inert solid supports on acceleration of alcohol fermentation by Saccharomyces cerevisiae was investigated. The enhanced rate of alcohol production was dependent on the nature of the support as well as on the amount used. Among all the tested supports, chitosan flakes showed the maximum yield of alcohol (93% of theoretical yield). This higher rate of alcohol production was associated with the twofold increase in the activity of alcohol dehydrogenase over control. Our results suggest that the addition of a small fraction of solids in submerged fermentations to facilitate cell anchorage for enhanced metabolic activity is easier and more economical compared to cell immobilization processes.
研究了各种惰性固体载体对酿酒酵母加速酒精发酵的催化作用。酒精产量的提高速率取决于载体的性质以及所用的量。在所有测试的载体中，壳聚糖薄片显示出最高的酒精产量（理论产量的93%）。这种较高的酒精生产速率与酒精脱氢酶活性比对照提高两倍有关。我们的结果表明，与细胞固定化过程相比，在深层发酵中添加一小部分固体以促进细胞固定以增强代谢活性更容易且更经济。
