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J Assist Reprod Genet. 2000 Jan;17(1):67-73. doi: 10.1023/a:1009458215882.
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mos proto-oncogene function.mos原癌基因功能。
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Essential role of germinal vesicle material in the meiotic cell cycle of Xenopus oocytes.生发泡物质在非洲爪蟾卵母细胞减数分裂细胞周期中的重要作用。
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Rescue of mouse embryos from 2-cell blocks by microinjection of maturation promoting factor.通过显微注射成熟促进因子挽救处于二细胞阻滞阶段的小鼠胚胎。
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本文引用的文献

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Regulation of Metaphase by a Maturation-Promoting Factor: (meiosis/mitosis/cell cycle/metaphase/maturation-promoting factor).成熟促进因子对中期的调控：（减数分裂/有丝分裂/细胞周期/中期/成熟促进因子）
Dev Growth Differ. 1988 Apr;30(2):105-115. doi: 10.1111/j.1440-169X.1988.00105.x.
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Intracytoplasmic sperm injection in the mouse.
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Nuclear maturation in pig and rabbit oocytes after interspecific fusion.种间融合后猪和兔卵母细胞的核成熟
Exp Cell Res. 1983 Jun;146(1):212-8. doi: 10.1016/0014-4827(83)90341-5.
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Oocyte maturation inhibitor, inhibin and steroid concentrations in porcine follicular fluid at various stages of the oestrous cycle.发情周期各阶段猪卵泡液中卵母细胞成熟抑制剂、抑制素和类固醇的浓度。
J Reprod Fertil. 1983 May;68(1):247-52. doi: 10.1530/jrf.0.0680247.
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Maturation-promoting factor induces nuclear envelope breakdown in cycloheximide-arrested embryos of Xenopus laevis.成熟促进因子可诱导非洲爪蟾在放线菌酮阻滞的胚胎中出现核膜破裂。
J Cell Biol. 1983 Jul;97(1):81-91. doi: 10.1083/jcb.97.1.81.
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Cytoplasmic control of nuclear behavior during meiotic maturation of frog oocytes.蛙卵母细胞减数分裂成熟过程中细胞核行为的细胞质调控
J Exp Zool. 1971 Jun;177(2):129-45. doi: 10.1002/jez.1401770202.
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The rôle of cumulus cells and serum in mouse oocyte maturation in vitro.卵丘细胞和血清在小鼠卵母细胞体外成熟中的作用。
J Reprod Fertil. 1973 Aug;34(2):241-5. doi: 10.1530/jrf.0.0340241.
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Gonadotropin action on cultured Graafian follicles: induction of maturation division of the mammalian oocyte and differentiation of the luteal cell.
Recent Prog Horm Res. 1974;30(0):79-138. doi: 10.1016/b978-0-12-571130-2.50007-9.
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Inhibitory effect of dibutyryl cAMP on mouse oocyte maturation in vitro.二丁酰环磷腺苷对小鼠卵母细胞体外成熟的抑制作用。
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Active maturation-promoting factor is present in mature mouse oocytes.活性促成熟因子存在于成熟的小鼠卵母细胞中。
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显微注射成熟促进因子（MPF）诱导小鼠卵母细胞体外成熟的研究。

Study of the in vitro maturation of mouse oocytes induced by microinjection of maturation promoting factor (MPF).

作者信息

Nakano H, Kubo H

机构信息

First Department of Obstetrics and Gynecology, Toho University School of Medicine, Tokyo, Japan.

出版信息

J Assist Reprod Genet. 2000 Jan;17(1):67-73. doi: 10.1023/a:1009458215882.

DOI:10.1023/a:1009458215882
PMID:10754786
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3455188/
Abstract

PURPOSE

Maturation promoting factor (MPF) acts at the resumption of meiosis and nonspecifically throughout the animal species. There exists a considerable body of literature on MPF, but little work has been done to study the induction of maturation of mammalian oocytes by microinjection of extracted MPF.

METHODS

Immature (GV-stage) mouse oocytes were microinjected MPF extracted from matured Xenopus eggs in the presence of dbcAMP.

RESULTS

The rate of germinal vesicle, breakdown (GVBD) induced at 24 hr after MPF injection was significantly higher (90.5%) than that of the control (2.2%), which was injected with HTF medium containing dbcAMP (P < 0.0001). The rate of extrusion of the first polar body at 24 hr after MPF injection was significantly higher (84.1%) than that of the same control (1.1%) (P < 0.0001).

CONCLUSIONS

From these results, it is concluded that the maturation of mammalian oocytes can be induced by the microinjection of MPF extracted from other species.

摘要

目的

成熟促进因子（MPF）在减数分裂恢复时发挥作用，且在整个动物物种中具有非特异性。关于MPF已有大量文献，但通过显微注射提取的MPF来研究哺乳动物卵母细胞成熟诱导的工作做得很少。

方法

在存在二丁酰环磷腺苷（dbcAMP）的情况下，将从成熟非洲爪蟾卵中提取的MPF显微注射到未成熟（生发泡期）小鼠卵母细胞中。

结果

MPF注射后24小时诱导的生发泡破裂（GVBD）率显著高于对照组（90.5%对比2.2%），对照组注射的是含有dbcAMP的HTF培养基（P < 0.0001）。MPF注射后24小时第一极体排出率显著高于同一对照组（84.1%对比1.1%）（P < 0.0001）。

结论

从这些结果可以得出结论，通过显微注射从其他物种提取的MPF可诱导哺乳动物卵母细胞成熟。