Fulka J
Exp Cell Res. 1983 Jun;146(1):212-8. doi: 10.1016/0014-4827(83)90341-5.
Porcine ovarian oocytes were fused with either homologous (porcine) or heterologous (rabbit) oocytes, both at different stages of maturation. The maturation-promoting factor (MPF) present in maturing porcine oocytes or ovulated rabbit oocytes induced rapid chromosome condensation of the oocytes with intact germinal vesicles (GVs). In the case of activation of ovulated rabbit oocyte, germinal vesicle breakdown (GVBD) of porcine oocytes was incomplete or did not occur. In the giant cells consisting of two immature porcine oocytes, meiotic maturation proceeded in the same manner as in unfused oocytes. However, in cells derived from fusion of immature porcine and rabbit oocytes, two metaphase groups of chromosomes were observed 6 h after fusion. It may be concluded that GVBD is governed after fusion by the cytoplasm originating from the oocytes of more advanced stages of maturation or from those which mature faster.
将处于不同成熟阶段的猪卵巢卵母细胞与同源（猪）或异源（兔）卵母细胞融合。成熟猪卵母细胞或排卵兔卵母细胞中存在的成熟促进因子（MPF）可诱导具有完整生发泡（GVs）的卵母细胞快速染色体凝聚。在排卵兔卵母细胞激活的情况下，猪卵母细胞的生发泡破裂（GVBD）不完全或未发生。在由两个未成熟猪卵母细胞组成的巨细胞中，减数分裂成熟过程与未融合卵母细胞相同。然而，在由未成熟猪卵母细胞和兔卵母细胞融合产生的细胞中，融合后6小时观察到两个中期染色体组。可以得出结论，GVBD在融合后由来自成熟阶段更高级或成熟更快的卵母细胞的细胞质控制。
