Pacios Bras C, Jordá M A, Wijfjes A H, Harteveld M, Stuurman N, Thomas-Oates J E, Spaink H P
Leiden University, Institute of Molecular Plant Sciences, The Netherlands.
Mol Plant Microbe Interact. 2000 Apr;13(4):475-9. doi: 10.1094/MPMI.2000.13.4.475.
Heterologous expression of NodZ and NolL proteins in Rhizobium leguminosarum bv. viciae led to the production of acetyl fucosylated lipo-chitin oligosaccharides (LCOs), indicating that the NolL protein obtained from Mesorhizobium loti functions as an acetyl transferase. We show that the NolL-dependent acetylation is specific for the fucosyl penta-N-acetylglucosamine species. In addition, the NolL protein caused elevated production of LCOs. Efficient nodulation of Lotus japonicus by the NodZ/NolL-producing strain was demonstrated. Nodulation efficiency was further improved by the addition of the ethylene inhibitor L-alpha-(2-aminoethoxyvinyl) glycine (AVG).
在豌豆根瘤菌蚕豆生物变种中异源表达NodZ和NolL蛋白导致了乙酰化岩藻糖基化脂壳寡糖（LCOs）的产生，这表明从百脉根中获得的NolL蛋白起着乙酰转移酶的作用。我们发现NolL依赖的乙酰化作用对岩藻糖基化五-N-乙酰葡糖胺种类具有特异性。此外，NolL蛋白导致LCOs产量增加。已证实产生NodZ/NolL的菌株能高效结瘤日本百脉根。通过添加乙烯抑制剂L-α-（2-氨基乙氧基乙烯基）甘氨酸（AVG），结瘤效率进一步提高。
