Yan Y, Zhang G, Chen C, Li X, Li Q
Institute of Animal and Veterinary Sciences, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China.
Res Vet Sci. 2000 Apr;68(2):115-8. doi: 10.1053/rvsc.1999.0343.
The full length of gene encoding Fc receptor types III for bovine immunoglobulin G (boFcgammaRIII, CD16) was obtained with PCR from the c DNA library of bovine alveolar macrophages. Through molecular cloning and sequencing it is shown that the coding gene has 507 bp and codes for 168 amino acids. There are signal peptide, transmembrane spanning domain and three potential N-linked glycosylation sites in the sequence. Compared with the boFcgammaRIII cDNA sequence cloned by Collins et al, it deletes 82 amino acids and has only one extracellular domain. It is suggested that this cDNA fragment is a special form of the gene structure of bovine FcgammaRIIIA (CD16-II).
从牛肺泡巨噬细胞的cDNA文库中通过聚合酶链反应（PCR）获得了编码牛免疫球蛋白G的III型Fc受体（boFcγRIII，CD16）的全长基因。通过分子克隆和测序表明，该编码基因有507个碱基对，编码168个氨基酸。该序列中有信号肽、跨膜结构域和三个潜在的N-糖基化位点。与Collins等人克隆的boFcγRIII cDNA序列相比，它缺失了82个氨基酸，仅具有一个细胞外结构域。提示该cDNA片段是牛FcγRIIIA（CD16-II）基因结构的一种特殊形式。
