Bailey S R, Andrews M J, Elliott J, Cunningham F
Department of Veterinary Basic Sciences, The Royal Veterinary College, North Mymms, Hatfield, Hertfordshire, AL9 7TA.
Res Vet Sci. 2000 Apr;68(2):175-80. doi: 10.1053/rvsc.1999.0356.
Platelets are thought to play a role in equine diseases such as acute laminitis and verminous arteritis and may be involved in allergic disease. Mediators implicated in the pathophysiology of these conditions activate platelets and responses may be enhanced by interactions between mediators. The present study compared platelet aggregation, thromboxane production and release of radiolabelled [(3)H]5- HT in response to 5- HT, histamine, ADP and PAF alone and in combination in vitro.PAF caused concentration-related aggregation, [(3)H]5- HT release and thromboxane production. In contrast, ADP caused aggregation and 5- HT induced the release of [(3)H]5- HT with little effect on other platelet functions. Histamine had little or no effect on equine platelets. Addition of 5- HT (10 microM) prior to ADP significantly displaced the aggregation response curve to the left. The profile of responses to PAF, ADP and 5- HT suggests differential activation of intracellular signalling pathways regulating these events. The enhanced response to ADP in the presence of 5- HT may have implications in thromboembolic disease in the horse.
血小板被认为在马的疾病如急性蹄叶炎和蠕虫性动脉炎中起作用，并且可能参与过敏性疾病。与这些病症的病理生理学相关的介质会激活血小板，并且介质之间的相互作用可能会增强反应。本研究比较了体外单独及联合使用5-羟色胺（5-HT）、组胺、二磷酸腺苷（ADP）和血小板活化因子（PAF）时血小板的聚集、血栓素生成以及放射性标记的[³H]5-HT的释放情况。PAF引起浓度相关的聚集、[³H]5-HT释放和血栓素生成。相比之下，ADP引起聚集，5-HT诱导[³H]5-HT释放，对其他血小板功能影响很小。组胺对马血小板几乎没有影响。在ADP之前加入5-HT（10微摩尔）可使聚集反应曲线显著向左移动。对PAF、ADP和5-HT的反应情况表明调节这些事件的细胞内信号通路存在差异激活。在5-HT存在下对ADP的增强反应可能对马的血栓栓塞性疾病有影响。
