绵羊（Ovis aries）染色体中的姐妹染色单体交换

Sister chromatid exchange in chromosomes of sheep (Ovis aries).

作者信息

Di Meo G P, Perucatti A, Fornataro D, Incarnato D, Ferrara L, Matassino D, Iannuzzi L

机构信息

National Research Council (CNR), Instituto di Ricerche Sull'Adattamento dei Bovini e dei Bufali all' Ambiente del Mezzogiorno (IABBAM), Naples, Italy.

出版信息

Cytobios. 2000;101(397):71-8.

PMID:10756979

Abstract

Blood lymphocyte cultures from 32 Comisana and Laticauda sheep breeds (15 males and 17 females) raised in Southern Italy were studied using sister chromatid exchange (SCE) test. Of the 932 cells studied, the SCE-mean value was 7.20 +/- 2.5 per cell for both breeds. Indeed, the SCE mean values were 7.12 +/- 2.45 and 7.28 +/- 2.55 in Comisana and Laticauda breeds, respectively, and the differences were not significant. No statistical differences were noticed between male and female cells (7.25 +/- 2.39 and 7.16 +/- 2.60, respectively). The SCE frequency distribution did not follow a Poisson distribution. The number of SCE were significantly higher than expected in chromosomes 1, 2 and 3 (p < 0.001) and significantly lower than expected in the X and remaining chromosomes (p < 0.001) on the basis of relative chromosome lengths.

摘要

利用姐妹染色单体交换（SCE）试验，对意大利南部饲养的32只科米萨纳羊和拉蒂考达羊（其中15只为公羊，17只为母羊）的血液淋巴细胞培养物进行了研究。在所研究的932个细胞中，两个品种的SCE平均值均为每个细胞7.20±2.5。实际上，科米萨纳羊和拉蒂考达羊的SCE平均值分别为7.12±2.45和7.28±2.55，差异不显著。在雄性和雌性细胞之间未观察到统计学差异（分别为7.25±2.39和7.16±2.60）。SCE频率分布不遵循泊松分布。根据相对染色体长度，1号、2号和3号染色体上的SCE数量显著高于预期（p<0.001），而X染色体和其余染色体上的SCE数量显著低于预期（p<0.001）。