Bozsakyová E, Wsólová L, Chalupa I
Cancer Research Institute of Slovak Academy of Sciences, Bratislava, Slovak Republic.
Int J Radiat Biol. 2005 Feb;81(2):177-85. doi: 10.1080/09553000500077120.
The aim was to investigate whether there are differences in the spontaneous and gamma-ray-induced genomic instability in peripheral blood lymphocytes between untreated cervical cancer patients and healthy women using the sister chromatid exchange (SCE) assay as an indicator of chromosomal instability. Lymphocyte cultures from whole venous blood of 10 patients with cervical neoplasia and 10 healthy female volunteers were cultivated in vitro and irradiated using a 60Co-gamma source. Slides were prepared using the standard air-drying procedure and stained by the fluorescence-plus Giemsa (FPG) technique. The number of SCE and the number of chromosomes were assessed in second-division metaphases. A radiation dose-dependent increase of SCE/cell and SCE/chromosome values were found in healthy women as well as in patients, while statistical analysis has shown significantly higher SCE frequencies in healthy women as compared with patients. Cellular kinetics expressed as replication indices (RI) calculated from the frequency of cells in first cell division (M1), second cell division (M2) and third cell division (M3) were also significantly different, while observed RI were higher for patients than for control individuals. The results suggest that patients with carcinoma of the cervix uteri have chromosomal stability changes reflected in statistically different levels of spontaneous and induced SCE in comparison with healthy individuals. Despite the unknown mechanisms of SCE formation, it is felt that the changed SCE frequency, especially after mutagen treatment, may be used as a marker of increased cancer risk.
目的是通过姐妹染色单体交换（SCE）试验作为染色体不稳定的指标，研究未经治疗的宫颈癌患者与健康女性外周血淋巴细胞中自发和γ射线诱导的基因组不稳定是否存在差异。从10例宫颈肿瘤患者和10名健康女性志愿者的全静脉血中获取淋巴细胞培养物，进行体外培养，并用60Co-γ源进行照射。使用标准空气干燥程序制备玻片，并用荧光加吉姆萨（FPG）技术染色。在第二次分裂中期评估SCE的数量和染色体的数量。在健康女性和患者中均发现SCE/细胞和SCE/染色体值随辐射剂量呈依赖性增加，而统计分析表明，与患者相比，健康女性的SCE频率显著更高。根据第一次细胞分裂（M1）、第二次细胞分裂（M2）和第三次细胞分裂（M3）中的细胞频率计算得出的以复制指数（RI）表示的细胞动力学也存在显著差异，而观察到的患者RI高于对照个体。结果表明，与健康个体相比，子宫颈癌患者的染色体稳定性变化反映在自发和诱导的SCE的统计学不同水平上。尽管SCE形成的机制尚不清楚，但人们认为，尤其是在诱变处理后，SCE频率的变化可能用作癌症风险增加的标志物。
