Cameron C E, Lukehart S A, Castro C, Molini B, Godornes C, Van Voorhis W C
Department of Medicine, University of Washington, Seattle, WA 98195, USA.
J Infect Dis. 2000 Apr;181(4):1401-13. doi: 10.1086/315399. Epub 2000 Apr 13.
By means of a differential screening technique, a 92-kDa antigen, designated Tp92, was identified from Treponema pallidum subspecies pallidum. This protein is similar in sequence to the protective surface antigens D15 from Haemophilus influenzae and Oma87 from Pasteurella multocida. Amino acid sequence analyses revealed a cleavable N-terminal signal sequence and predicted the outer membrane location for Tp92. In support of this, antiserum raised against recombinant Tp92 promotes opsonization and phagocytosis of T. pallidum by rabbit macrophages, and anti-Tp92 reactivity is absent from washed treponemal preparations presumed to be lacking outer membranes. The Tp92 amino acid sequence is 95.5%-100% conserved among 11 strains representing 4 pathogenic treponemes, and immunization with recombinant Tp92 partially protected rabbits from subsequent T. pallidum challenge. These results demonstrate that Tp92 is an invariant, immunoprotective antigen that may be present on the surface of T. pallidum and may represent a potential vaccine candidate for syphilis.
通过差异筛选技术，从梅毒螺旋体苍白亚种中鉴定出一种92 kDa的抗原，命名为Tp92。该蛋白在序列上与流感嗜血杆菌的保护性表面抗原D15和多杀巴斯德菌的Oma87相似。氨基酸序列分析揭示了一个可切割的N端信号序列，并预测Tp92位于外膜。支持这一观点的是，针对重组Tp92产生的抗血清可促进兔巨噬细胞对梅毒螺旋体的调理吞噬作用，而假定缺乏外膜的梅毒螺旋体制剂中不存在抗Tp92反应性。在代表4种致病性密螺旋体的11个菌株中，Tp92氨基酸序列的保守性为95.5%-100%，用重组Tp92免疫可使兔子部分免受随后梅毒螺旋体攻击的影响。这些结果表明，Tp92是一种不变的免疫保护性抗原，可能存在于梅毒螺旋体表面，可能是梅毒潜在的疫苗候选物。
