Stienstra S, Peeters T, van der Straaten A M, Kadir N
Red Cross Blood Bank Nijmegen e.o., The Netherlands.
Beitr Infusionsther. 1992;30:85-91.
A new ELISA test system for screening antibodies directed against Treponema pallidum is presented. The ELISA is based on a recombinant DNA-derived T. pallidum membrane protein A (TmpA). It makes the production of the kit easier as it abandons the necessity to culture T. pallidum in living animals. The specificity and sensitivity of the ELISA are comparable with those of the TPHA and FTA-ABS. The test gives only a positive result when there are antibodies against the TmpA antigen and, therefore, it gives in screening (e.g. blood donors) no false-positive reaction in persons who, due to an old well-treated infection, have a so-called serological scar. As these donors are noninfective, they should be concerned as negative in syphilis screening.
介绍了一种用于筛查抗梅毒螺旋体抗体的新型酶联免疫吸附测定（ELISA）检测系统。该ELISA基于重组DNA衍生的梅毒螺旋体膜蛋白A（TmpA）。它摒弃了在活体动物中培养梅毒螺旋体的必要性，从而使试剂盒的生产更加简便。ELISA的特异性和敏感性与梅毒螺旋体血凝试验（TPHA）和荧光密螺旋体抗体吸收试验（FTA-ABS）相当。该检测仅在存在针对TmpA抗原的抗体时给出阳性结果，因此，在筛查（如献血者）时，对于因既往感染得到良好治疗而有所谓血清学瘢痕的人不会产生假阳性反应。由于这些献血者没有传染性，在梅毒筛查中应判定为阴性。
