Segerson E C, Beetham P K
Department of Animal Science, North Carolina A&T State University, Greensboro 27411, USA.
J Anim Sci. 2000 Mar;78(3):709-17. doi: 10.2527/2000.783709x.
Numbers of fluorescein isothiocyanate (FITC)-labeled bone marrow (BM) cells of donor lambs were quantified within endometrial cell suspensions following their administration to ovariectomized (OVX; control-and estradiol-17beta-treated) and intact (estrus, d-14 cyclic and pregnant) ewes. The numbers of fluorescent BM cells were greater (P < .05) for the estrous and d-14 cyclic ewes than for both groups of OVX ewes. Fractionation of the endometrial cells with Percoll revealed that the majority of fluorescent cells were low-density (1.002 to 1.056 g/mL) cells. In coculture experiments, low-density cells from lamb BM not only suppressed the incorporation of thymidine into phytohemagglutinin-treated peripheral blood lymphocytes, but the cells also released suppressor factor into the culture medium. Suppressor activity tended to be reversed (P < .1) by a pan-specific neutralization antibody to transforming growth factor-beta (TGF-beta); however, the activity was unaffected by a neutralization antibody to TGF-beta2. These findings suggest that ovine endometrial suppressor cells may represent a population of low-density BM-derived natural suppressor cells, and their trafficking and localization patterns may depend on an ovarian factor(s). Further, suppressor activity does not seem to be mediated by TGF-beta2.
将异硫氰酸荧光素（FITC）标记的供体羔羊骨髓（BM）细胞注入去卵巢（OVX；对照组和经17β-雌二醇处理）以及完整（发情期、周期第14天和怀孕）的母羊体内后，对子宫内膜细胞悬液中的此类细胞数量进行了定量分析。发情期和周期第14天的母羊体内荧光BM细胞的数量比两组去卵巢母羊中的荧光BM细胞数量更多（P < 0.05）。用Percoll对子宫内膜细胞进行分级分离显示，大多数荧光细胞是低密度（1.002至1.056 g/mL）细胞。在共培养实验中，来自羔羊骨髓的低密度细胞不仅抑制了胸腺嘧啶核苷掺入经植物血凝素处理的外周血淋巴细胞，而且这些细胞还向培养基中释放了抑制因子。针对转化生长因子-β（TGF-β）的泛特异性中和抗体倾向于逆转抑制活性（P < 0.）；然而，该活性不受针对TGF-β2的中和抗体的影响。这些发现表明，绵羊子宫内膜抑制细胞可能代表了一群源自骨髓的低密度天然抑制细胞，其运输和定位模式可能取决于一种或多种卵巢因子。此外，抑制活性似乎不是由TGF-β2介导的。
