Segerson E C, Li H, Talbott C W
Department of Animal Science, North Carolina A&T State University, Greensboro 27411, USA.
J Anim Sci. 1997 Oct;75(10):2778-87. doi: 10.2527/1997.75102778x.
We evaluated the regulation of ovine uterine (UT) suppressor cell activity by progesterone (P4), estradiol-17 beta (E2), and P4 + E2 in ovariectomized (OVX) ewes. Following 14 d of steroid injections, endometrial cells (designated as UT cells) were recovered postmortem, and unfractionated and fractionated cells were assessed for suppression of autologous phytohemagglutinin (PHA)-treated peripheral blood lymphocytes (PBL). Supernatants from cultured UT cells were also assessed for suppressor activity. In other experiments, UT cells recovered from nontreated OVX ewes were cocultured with PHA-treated PBL and varying concentrations (1 x 10(-11) to 1 x 10(-5) M) of each steroid preparation. Supernatants from separate cultures that contained UT cells and steroids were evaluated for suppressor activity. Uterine cells from control and steroid-treated ewes suppressed proliferative responses of PHA-treated PBL; however, suppressor activity of UT cells was greater (P < .05) for E2-treated than for control and P4-treated ewes. Uterine suppressor cells from steroid-treated ewes sedimented in Percoll within a density range of 1.002 to 1.056 g/mL. Uterine cells from all ewes released suppressor factor(s) into the culture medium; however, the activity of the supernatant from the cultured cells was not increased for the steroid-treated ewes. For cocultures that contained steroids and cultures that contained supernatant, suppressor activity of the UT cells was increased by specific concentrations of each steroid preparation. These findings demonstrate that reproductive steroids augment ovine UT suppressor cell activity.
我们评估了孕酮（P4）、17β-雌二醇（E2）以及P4 + E2对去卵巢（OVX）母羊子宫（UT）抑制细胞活性的调节作用。在进行14天的类固醇注射后，于死后采集子宫内膜细胞（称为UT细胞），并对未分级和分级的细胞进行自体植物血凝素（PHA）处理的外周血淋巴细胞（PBL）抑制作用评估。还对培养的UT细胞的上清液进行了抑制活性评估。在其他实验中，将从未经处理的OVX母羊中回收的UT细胞与PHA处理的PBL以及每种类固醇制剂的不同浓度（1×10⁻¹¹至1×10⁻⁵ M）共同培养。对含有UT细胞和类固醇的单独培养物的上清液进行抑制活性评估。来自对照和类固醇处理母羊的子宫细胞抑制了PHA处理的PBL的增殖反应；然而，E2处理的UT细胞的抑制活性比对照和P4处理的母羊更高（P <.05）。来自类固醇处理母羊的子宫抑制细胞在Percoll中的沉降密度范围为1.002至1.056 g/mL。所有母羊的子宫细胞都向培养基中释放了抑制因子；然而，类固醇处理母羊的培养细胞上清液的活性并未增加。对于含有类固醇的共培养物和含有上清液的培养物，每种类固醇制剂的特定浓度均增加了UT细胞的抑制活性。这些发现表明，生殖类固醇增强了绵羊UT抑制细胞的活性。
