Misof B, Anderson C L, Hadrys H
Institute for Evolutionary Biology and Ecology, University of Bonn, An der Immenburg 1, Bonn, D-53121, Germany.
Mol Phylogenet Evol. 2000 Apr;15(1):5-14. doi: 10.1006/mpev.1999.0724.
We seek to reconstruct the phylogenetic relationships of the damselfly genus Calopteryx, for which extensive behavioral and morphological knowledge already exists. To date, analyses of the evolutionary pathways of different life history traits have been hampered by the absence of a robust phylogeny based on morphological data. In this study, we concentrate on establishing phylogenetic information from parts of the 16S rDNA gene, which we sequenced for nine Calopteryx species and five outgroup species. The mt 16S rDNA data set did not show signs of saturated variation for ingroup taxa, and phylogenetic reconstructions were insensitive to variation of outgroup taxa. Parsimony, neighbor-joining, and maximum-likelihood reconstructions agreed on parts of the tree. A consensus tree summarizes the significant results and indicates problematic nodes. The 16S rDNA sequences support monophyly of the genera Mnais, Matrona, and Calopteryx. However, the genus Calopteryx may not be monophyletic, since Matrona basilaris and Calopteryx atrata are sister taxa under every parameter setting. The North American and European taxa each appear as monophyletic clades, while the Asian Calopteryx atrata and Calopteryx cornelia are not monophyletic. Our data implies a different paleobiogeographic history of the Eurasian and North American species, with extant Eurasian species complexes shaped by glacial periods, in contrast to extant North American species groups.
我们试图重建豆娘属Calopteryx的系统发育关系，对于该属我们已经有了广泛的行为学和形态学知识。迄今为止，由于缺乏基于形态学数据的可靠系统发育树，不同生活史特征的进化途径分析受到了阻碍。在本研究中，我们专注于从16S rDNA基因的部分序列中建立系统发育信息，我们对9种Calopteryx物种和5种外类群物种进行了该基因的测序。线粒体16S rDNA数据集对于内类群分类单元没有显示出饱和变异的迹象，并且系统发育重建对外类群分类单元的变异不敏感。简约法、邻接法和最大似然法重建在部分树结构上达成了一致。一棵共识树总结了重要结果并指出了有问题的节点。16S rDNA序列支持Mnais属、Matrona属和Calopteryx属的单系性。然而，Calopteryx属可能不是单系的，因为在每种参数设置下，Matrona basilaris和Calopteryx atrata都是姐妹分类单元。北美和欧洲的分类单元各自呈现为单系分支，而亚洲的Calopteryx atrata和Calopteryx cornelia不是单系的。我们的数据意味着欧亚和北美物种有着不同的古生物地理历史，与现存北美物种群不同，现存欧亚物种复合体是由冰川期塑造的。
