McPheron B A, Han H Y
Department of Entomology and Institute of Molecular Evolutionary Genetics, Pennsylvania State University, University Park 16802-3508, USA.
Mol Phylogenet Evol. 1997 Feb;7(1):1-16. doi: 10.1006/mpev.1996.0369.
Partial sequences (approximately 850 bp) of the mitochondrial 16S ribosomal RNA gene were determined for 21 members of the fruit fly genus Rhagoletis and 6 related tephritid taxa by sequencing DNA amplified by the polymerase chain reaction. Sequences were highly A + T rich, with an average G + C content of 19.2%. Sequence divergence ranged from 0 to 11.7% among the included taxa. Sequences were used to reconstruct the phylogeny of this group of flies with neighbor-joining and maximum parsimony methods. A group of 18 North American Rhagoletis species formed a monophyletic clade, and three morphologically based species groups were identified with strong statistical support. Relationships among the species groups were partially resolved. The tabellaria species group appears to be paraphyletic with respect to the pomonella species group, in contrast to previous allozyme analyses that united the cingulata and pomonella groups. The relations of the North American clade to other genera within the tribe Carpomyini were not clearly resolved. Rhagoletis striatella, a North American species that uses members of the tomato family (Solanaceae) as a larval host, may be more closely associated with South American Rhagoletis species and related genera than with the other North American Rhagoletis species.
通过对聚合酶链反应扩增的DNA进行测序，测定了果蝇属Rhagoletis的21个成员以及6个相关实蝇类群的线粒体16S核糖体RNA基因的部分序列（约850 bp）。序列富含A + T，平均G + C含量为19.2%。在所包含的类群中，序列分歧度在0至11.7%之间。利用邻接法和最大简约法，用这些序列重建了这组果蝇的系统发育。18种北美Rhagoletis物种形成了一个单系类群，并且在强大的统计支持下识别出了三个基于形态学的物种组。物种组之间的关系得到了部分解析。与之前将cingulata和pomonella组合并的等位酶分析相反，tabellaria物种组相对于pomonella物种组似乎是并系的。北美类群与Carpomyini族内其他属的关系尚未明确解析。北美物种Rhagoletis striatella以茄科植物为幼虫宿主，它与南美Rhagoletis物种及相关属的关系可能比与其他北美Rhagoletis物种的关系更为密切。
