Artiss T, Schultz T R, Polhemus D A, Simon C
Department of Biology, Clark University, 950 Main Street, Worcester, Massachusetts, 01610-1477, USA.
Mol Phylogenet Evol. 2001 Mar;18(3):348-61. doi: 10.1006/mpev.2000.0867.
Molecular phylogenetic relationships among members of the odonate genus Libellula (Odonata: Anisoptera: Libellulidae) were examined using 735 bp of mitochondrial COI and 416 bp of 16S ribosomal RNA gene sequences. Considerable debate exists over several relationships within Libellula, as well over the status of two putative genera often placed as subgenera within Libellula: Ladona and Plathemis. Parsimony and maximum-likelihood analyses of the separate and combined data sets indicate that Plathemis is basal and monophyletic and that Ladona is the sister clade to the remainder of Libellula sensu stricto (s.s.) (all species within the genus Libellula, excluding Plathemis and Ladona). Moreover, two European taxa, Libellula fulva and L. depressa, were found to occupy a sister group relationship within the Ladona clade. Relationships within Libellula s.s. are less well resolved. However, monophyletic lineages within the genus are largely consistent with morphologically based subgeneric classifications. Although tree topologies from each analysis differed in some details, the differences were in no case statistically significant. The analysis of the combined COI and 16S data yielded trees with overall stronger support than analyses of either gene alone. Several analyses failed to support the monophyly of Libellula sensu lato due to the inclusion of one or more outgroup species. However, statistical comparisons of topologies produced by unconstrained analyses and analyses in which the monophyly of Libellula was constrained indicate that any differences are nonsignificant. Based on morphological data, we therefore reject the paraphyly of Libellula and accept the outgroup status of Orthemis ferruginea and Pachydiplax longipennis.
利用线粒体细胞色素氧化酶亚基I（COI）的735个碱基对和16S核糖体RNA基因序列的416个碱基对，研究了蜻蛉属（蜻蜓目：差翅亚目：蜻蛉科）成员之间的分子系统发育关系。蜻蛉属内的几种关系存在相当大的争议，同时对于通常被置于蜻蛉属亚属地位的两个假定属：多纹蜻属和宽痣蜻属的地位也存在争议。对单独和合并数据集进行的简约分析和最大似然分析表明，宽痣蜻属是基部类群且为单系类群，多纹蜻属是狭义蜻蛉属（蜻蛉属内所有物种，不包括宽痣蜻属和多纹蜻属）其余部分的姐妹分支。此外，发现两个欧洲分类单元，即黄蜻和方带黄蜻，在多纹蜻分支内占据姐妹群关系。狭义蜻蛉属内的关系解析度较低。然而，该属内的单系谱系在很大程度上与基于形态学的亚属分类一致。尽管每次分析得到的树拓扑结构在某些细节上有所不同，但这些差异在任何情况下都不具有统计学意义。对合并的COI和16S数据的分析产生的树，总体支持度比单独分析任何一个基因都更强。由于纳入了一个或多个外类群物种，几次分析都未能支持广义蜻蛉属的单系性。然而，对无约束分析和蜻蛉属单系性受约束分析所产生的拓扑结构进行的统计比较表明，任何差异都不显著。因此，基于形态学数据，我们拒绝蜻蛉属的并系性，并接受铁锈色斜痣蜻和长叶异痣蜻的外类群地位。
