Nishiyama N, Asakura T, Suzuki K, Komatsu K, Nemoto K
Department of Dental Materials, Nihon University School of Dentistry at Matsudo, Chiba, Japan.
J Dent Res. 2000 Mar;79(3):806-11. doi: 10.1177/00220345000790030401.
The application of the hydrophilic methacrylate primer to acid-etched dentin increases the bond strength of the resin to the acid-etched dentin. However, the mechanism for the bonding of the resin to dentinal collagen through the primer remains to be determined. Before a more effective adhesive primer can be developed, we must understand the adsorption behavior of the primer to the dentinal collagen. The purpose of this study was to determine how 5 derivatives of N-methacryloyl-omega-amino acid (NMomegaA) primers enhance the bonding of the resin to acid-etched dentin. The interaction between the NMomegaA primers and dentinal collagen was studied by the 13C NMR technique, including the observation of spin-lattice relaxation times, T1. When the dentinal collagen was dispersed into the NMomegaA solution, the T1 values of the two carbonyl carbons attributed to the amide and the carboxylic acid in the NMomegaA molecule decreased dramatically. This result was due to the interaction between the amide group and the carboxylic acid group in the NMomegaA molecule and the dentinal collagen molecule. The T1 values of these carbonyl carbons decreased when the number of methylene groups in the NMomegaA molecule increased. The interaction became stronger as the number of methylene groups in the NMomegaA molecule was increased. Further, the bond strength of the resin to the acid-etched dentin primed with NMomegaA increased with a decrease in the T1 value of the amide carbonyl carbon. The strength of the interaction of the NMomegaA primer to the dentinal collagen molecule, determined by the 13C NMR technique, showed a direct correlation with the bond strength of the resin to acid-etched dentin that was treated with the NMomegaA primer.
将亲水性甲基丙烯酸酯底漆应用于酸蚀牙本质可提高树脂与酸蚀牙本质之间的粘结强度。然而，通过底漆使树脂与牙本质胶原结合的机制仍有待确定。在开发出更有效的粘结底漆之前，我们必须了解底漆对牙本质胶原的吸附行为。本研究的目的是确定N-甲基丙烯酰基-ω-氨基酸（NMomegaA）底漆的5种衍生物如何增强树脂与酸蚀牙本质的粘结。通过13C NMR技术研究了NMomegaA底漆与牙本质胶原之间的相互作用，包括自旋晶格弛豫时间T1的观测。当牙本质胶原分散到NMomegaA溶液中时，NMomegaA分子中酰胺和羧酸的两个羰基碳的T1值显著降低。这一结果是由于NMomegaA分子中的酰胺基团和羧酸基团与牙本质胶原分子之间的相互作用。当NMomegaA分子中亚甲基的数量增加时，这些羰基碳的T1值降低。随着NMomegaA分子中亚甲基数量的增加，相互作用变得更强。此外，用NMomegaA预处理的酸蚀牙本质上树脂的粘结强度随着酰胺羰基碳T1值的降低而增加。通过13C NMR技术测定的NMomegaA底漆与牙本质胶原分子的相互作用强度与用NMomegaA底漆处理的酸蚀牙本质上树脂的粘结强度直接相关。
