Devarajan P V, Adani M H, Gandhi A S
Department of Chemical Technology, University of Mumbai, Matunga, India.
J Pharm Biomed Anal. 2000 May;22(4):685-90. doi: 10.1016/s0731-7085(99)00295-2.
A high performance thin layer chromatographic (HPTLC) method for the simultaneous quantification of lignocaine hydrochloride (LIG) and phenylephrine hydrochloride (PHE) is described. The mobile phase consisted of ethyl acetate-methanol ammonia (4:1:0.4 v/v/v). The densitometric determination of LIG and PHE was carried out at 262 nm and 291 nm, respectively. The calibration curves of LIG and PHE were linear in the range of 8-18 microg and 4-9 microg, respectively. The method was validated with respect to system precision, method precision, recoveries, intra-day and inter-day variation. The system was applied for the simultaneous determination of LIG and PHE from a new drug delivery system. The results indicate that the method is simple, specific, selective and reliable for simultaneous quantitative determination of LIG and PHE as bulk drug and from formulations.
描述了一种同时定量测定盐酸利多卡因（LIG）和盐酸去氧肾上腺素（PHE）的高效薄层色谱（HPTLC）方法。流动相由乙酸乙酯 - 甲醇 - 氨水（4:1:0.4 v/v/v）组成。LIG和PHE的密度测定分别在262 nm和291 nm处进行。LIG和PHE的校准曲线分别在8 - 18μg和4 - 9μg范围内呈线性。该方法在系统精密度、方法精密度、回收率、日内和日间变化方面进行了验证。该系统用于同时测定来自新型药物递送系统的LIG和PHE。结果表明，该方法对于同时定量测定原料药和制剂中的LIG和PHE而言简单、特异、选择性好且可靠。
