Salimova E, Sohrmann M, Fournier N, Simanis V
Cell Cycle Control Laboratory, ISREC, Chemin des Boveresses, 1066 Epalinges, Switzerland.
J Cell Sci. 2000 May;113 ( Pt 10):1695-704. doi: 10.1242/jcs.113.10.1695.
We have isolated the Schizosaccharomyces pombe orthologue of the Saccharomyces cerevisiae MOB1 gene in a screen designed to enrich for septation mutants. The gene is essential, and cells lacking it display a phenotype typical of septation signalling network mutants. mob1p is located on both spindle pole bodies throughout mitosis. In addition it is also co-localised with the medial ring later in mitosis, and flanks the septum as the medial ring contracts. We also demonstrate that mob1p can be precipitated from cells in a complex with the septation regulating kinase sid2p.
我们在一个旨在富集隔膜突变体的筛选中，分离出了酿酒酵母MOB1基因在粟酒裂殖酵母中的直系同源基因。该基因是必需的，缺乏它的细胞表现出隔膜信号网络突变体的典型表型。在整个有丝分裂过程中，mob1p定位于两个纺锤极体上。此外，在有丝分裂后期它还与中间环共定位，并且随着中间环收缩，它位于隔膜两侧。我们还证明，mob1p可以与调节隔膜的激酶sid2p形成复合物，从细胞中沉淀出来。
